Abstract Decolorization and removal of contaminants in textile wastewater are essential for pollution control. This study investigated the potential application of a halophilic and halotolerant Shewanella indica (S. indica) strain as immobilized biofilm in two attached growth bioreactors with moving bed bioreactor carrier and polyurethane porous gel carrier, respectively. Under optimized operation conditions, both bioreactors had high tolerance for Reactive Black 5 (RB5) concentration fluctuation and could decolorize RB5 effectively and stably (97% on average) for over 6 months. The decolorization of RB5 in bioreactors was mainly due to microbially mediated cleavage of the azo dye, though adsorption by biofilm and carrier (contribution of different carriers varied) also contributed to the process. However, the azo dye was barely mineralized, and the toxicity remained after decolorization. Overall, this study demonstrated the viability and sustainability of the bioreactors incubated with S. indica in decolorizing RB5. Nevertheless, further technologies need to be explored to detoxify the degradation metabolites.

中文翻译：

---

在不同载体上固定化 Shewanella indica 菌株的附着生长生物反应器中，微生物介导的 Reactive Black 5 裂解

摘要 纺织废水中污染物的脱色和去除对于污染控制至关重要。本研究调查了嗜盐和耐盐 Shewanella indica (S. indica) 菌株作为固定生物膜在两个分别带有移动床生物反应器载体和聚氨酯多孔凝胶载体的附着生长生物反应器中的潜在应用。在优化的操作条件下，两种生物反应器对活性黑 5 (RB5) 浓度波动具有较高的耐受性，并且可以有效稳定地（平均 97%）脱色 RB5 超过 6 个月。RB5 在生物反应器中的脱色主要是由于微生物介导的偶氮染料裂解，尽管生物膜和载体的吸附（不同载体的贡献不同）也促成了该过程。然而，偶氮染料几乎没有矿化，脱色后仍有毒性。总体而言，这项研究证明了与 S. indica 一起孵育的生物反应器在 RB5 脱色方面的可行性和可持续性。然而，需要探索进一步的技术来解毒降解代谢物。