Abstract Protein therapy has received significant importance in healthcare industry to combat a wide variety of diseases such as cancer, metabolic disorders, and autoimmune disease. However, the efficiency of in vitro and in vivo protein delivery is hindered by their protein instability and less life span. Herein, we prepared magnetic nanoparticles decorated with graphene oxide chitosan composite (Fe-GO-CS) as a nanocarrier for protein. Bovine serum albumin (BSA) is employed as a protein with the prepared nanocarrier to study its stability and activity. The structural and morphological analysis were carried out using XRD, FTIR, FESEM, VSM and Raman spectroscopy. SDS page analysis showed no remarkable change after exposing Fe-GO-BSA and Fe-GO-CS-BSA solution in trypsin after a time duration of 30 min and 3 h. The Fe-GO-CS exhibits a good drug loading and releasing profile when compared with Fe-GO composite and this nanocarrier protects the protein from enzymatic cleavage. Hence Fe-GO-CS composite is a better nanocarrier that can be applied for clinical applications practically.

中文翻译：

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磁性纳米粒子修饰的氧化石墨烯-壳聚糖复合材料作为有效的蛋白质递送纳米载体

摘要 蛋白质疗法在医疗保健行业具有重要意义，可用于对抗多种疾病，例如癌症、代谢紊乱和自身免疫性疾病。然而，体外和体内蛋白质递送的效率受到其蛋白质不稳定性和较短寿命的阻碍。在此，我们制备了用氧化石墨烯壳聚糖复合材料（Fe-GO-CS）装饰的磁性纳米粒子作为蛋白质的纳米载体。牛血清白蛋白 (BSA) 作为蛋白质与制备的纳米载体一起使用，以研究其稳定性和活性。使用XRD、FTIR、FESEM、VSM和拉曼光谱进行结构和形态分析。SDS 页分析显示，将 Fe-GO-BSA 和 Fe-GO-CS-BSA 溶液暴露在胰蛋白酶中 30 分钟和 3 小时后，没有显着变化。与 Fe-GO 复合材料相比，Fe-GO-CS 表现出良好的载药和释放特性，这种纳米载体可保护蛋白质免受酶促裂解。因此，Fe-GO-CS 复合材料是一种较好的纳米载体，可以实际应用于临床。