Tetracycline (TC) in soil severely imperils food security and ecosystem function. Metaphire guillelmi is a common species in farmland. It could impact the degradation of antibiotics. However, how it affects is rarely unknown. Hence, the present study aimed to investigate the effects of M. guillelmi on the TC degradation in soil and the changes of the antioxidant system and intestinal bacteria in M. guillelmi. The treatments that M. guillelmi was inoculated on soil contaminated with different TC concentrations were contrasted with those without M. guillelmi. After 21 days, the degradation rate of TC significantly increased by 13.70%, 18.14% and 29.01% at 10, 50 and 100 mg kg ^-1 TC dose, respectively, due to the inoculation of M. guillelmi. The half-life of TC was also shortened nearly by 1/3 to 2/3. Superoxide dismutase (SOD) increased in a dose-dependent manner with the increase of TC concentration on the 7^th and 14^th day. Catalase (CAT) and glutathione S-transferase (GST) presented an inverted U-shaped dose response on the 7^th day, and the peak of enzyme activities occurred at TC concentration of 0.1, 1 mg kg ^-1 (CAT) and 0.1 mg kg ^-1 (GST). Malondialdehyde (MDA) contents did not change significantly. At the phylum level, only Verrucomicrobia significantly decreased under 1 mg kg ^-1 and 100 mg kg ^-1 TC dose. Genus Paracoccus, Singulisphaera, Acinetobacter and Bacillus significantly increased and became the dominant bacterium during the TC degradation. Overall, the antioxidant system and intestinal bacteria of M. guillelmi were affected by the different concentrations of TC pollution, which provided new ideas for the research of mechanism of TC degradation by earthworms in the future.

土壤中的四环素严重危害粮食安全和生态系统功能。斜纹兰guillelmi是农田中的常见物种。它可能会影响抗生素的降解。但是，它如何影响鲜为人知。因此，本研究旨在探讨古吉尔氏菌对土壤TC降解的影响以及古吉尔氏菌中抗氧化系统和肠道细菌的变化。对比在没有TC污染的土壤中接种guillelmi污染土壤的处理方法。21天后，在10、50和100 mg kg ^-1时，TC的降解率显着提高了13.70％，18.14％和29.01％。TC剂量分别由于接种了guillelmi。TC的半衰期也缩短了近1/3至2/3。超氧化物歧化酶（SOD）以剂量依赖的方式与TC浓度对7的增加而增加^日和14^日的日子。过氧化氢酶（CAT）和谷胱甘肽S-转移酶（GST）呈现在7倒U形剂量响应^第一天，和酶活性的峰，在0.1 TC浓度，1毫克公斤发生^-1（CAT）和0.1mg千克^-1（GST）。丙二醛（MDA）含量没有明显变化。在门的水平上，在1 mg kg ^-1和100 mg kg ^-1以下仅Verrucomicrobia显着降低TC剂量。副球菌属，棘孢菌属，不动杆菌属和芽孢杆菌显着增加，并成为TC降解过程中的优势细菌。总体而言，不同浓度的TC污染会影响guillelmi菌的抗氧化系统和肠道细菌，这为今后研究degradation降解TC的机理提供了新思路。