The incidence of pancreatic neuroendocrine neoplasms (PanNENs) has gradually increased. PanNENs comprise two subtypes with different clinical manifestations and molecular mechanisms: functional PanNENs and nonfunctional PanNENs. Excessive hormones and tumor progression severely affect the quality of life of patients or are even life threatening. However, the molecular mechanisms of hormone secretion and tumor progression in PanNENs have not yet been fully elucidated. At present, advancements in sequencing technologies have led to the exploration of new biological markers and an advanced understanding of molecular mechanisms in PanNENs. Multiomics sequencing could reveal differences and similarities in molecular features in different fields. However, sequencing studies of PanNENs are booming and should be summarized to integrate the current findings. In this review, we summarize the current status of multiomics sequencing in PanNENs to further guide its application. We explore mainly advancements in the genome, transcriptome, and DNA methylation fields. In addition, the cell origin of PanNENs, which has been a hot issue in sequencing research, is described in multiple fields.

胰腺神经内分泌肿瘤（PanNENs）的发病率逐渐增加。PanNENs包含两个具有不同临床表现和分子机制的亚型：功能性PanNENs和非功能性PanNENs。过多的激素和肿瘤进展严重影响患者的生活质量，甚至危及生命。但是，尚未完全阐明PanNENs中激素分泌和肿瘤进展的分子机制。目前，测序技术的进步已导致对新的生物标记物的探索以及对PanNENs分子机制的高级理解。多元组学测序可以揭示不同领域分子特征的差异和相似性。然而，PanNENs的测序研究正在蓬勃发展，应加以总结以整合当前的发现。在这篇综述中，我们总结了PanNEN中多组学测序的现状，以进一步指导其应用。我们主要探索基因组，转录组和DNA甲基化领域的进展。此外，在多个领域描述了PanNENs的细胞起源，这是测序研究中的一个热门问题。