Organ-on-a-Chip (OOC) devices have seen major advances in the last years with respect to biological complexity, physiological composition and biomedical relevance. In this context, integration of vasculature has proven to be a crucial element for long-term culture of thick tissue samples as well as for realistic pharmacokinetic, toxicity and metabolic modelling. With the emergence of digital production technologies and the reinvention of existing tools, a multitude of design approaches for guided angio- and vasculogenesis is available today. The underlying production methods can be categorized into biosynthetic, biomimetic and self-assembled vasculature formation. The diversity and importance of production approaches, vascularization strategies as well as biomaterials and cell sourcing are illustrated in this work. A comprehensive technological review with a strong focus on the challenge of producing physiologically relevant vascular structures is given. Finally, the remaining obstacles and opportunities in the development of vascularized Organ-on-a-Chip platforms for advancing drug development and predictive disease modelling are noted.

近年来，芯片上器官（OOC）设备在生物学复杂性，生理成分和生物医学相关性方面取得了重大进步。在这种情况下，脉管系统整合已被证明是长期培养厚组织样品以及进行实际药代动力学，毒性和代谢建模的关键要素。随着数字化生产技术的出现和现有工具的重新发明，如今有许多用于指导血管和血管生成的设计方法可供使用。潜在的生产方法可以分为生物合成，仿生和自组装脉管系统形成。这项工作说明了生产方法，血管化策略以及生物材料和细胞来源的多样性和重要性。进行了全面的技术综述，重点关注产生生理相关的血管结构的挑战。最后，指出了血管化器官芯片平台开发中尚存的障碍和机遇，以促进药物开发和疾病预测模型的建立。