In recent years, a variety of imaging techniques operating at nanoscale resolution have been reported. These techniques have the potential to enrich our understanding of bacterial species relevant to human health, such as antibiotic-resistant pathogens. However, owing to the novelty of these techniques, their use is still confined to addressing very particular applications, and their availability is limited owing to associated costs and required expertise. Among these, scattering-type scanning near field optical microscopy (s-SNOM) has been demonstrated as a powerful tool for exploring important optical properties at nanoscale resolution, depending only on the size of a sharp tip. Despite its huge potential to resolve aspects that cannot be tackled otherwise, the penetration of s-SNOM into the life sciences is still proceeding at a slow pace for the aforementioned reasons.

中文翻译：

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SSNOMBACTER：细菌细胞的散射型扫描近场光学显微镜和原子力显微镜图像的集合


近年来，已经报道了多种在纳米级分辨率下运行的成像技术。这些技术有可能丰富我们对与人类健康相关的细菌物种的了解，例如抗生素耐药病原体。然而，由于这些技术的新颖性，它们的使用仍然局限于解决非常特殊的应用，并且由于相关的成本和所需的专业知识，它们的可用性受到限制。其中，散射型扫描近场光学显微镜（s-SNOM）已被证明是一种强大的工具，可以在纳米级分辨率下探索重要的光学特性，而该分辨率仅取决于尖锐尖端的尺寸。尽管 s-SNOM 具有解决其他方法无法解决的问题的巨大潜力，但由于上述原因，s-SNOM 向生命科学的渗透仍然进展缓慢。