SummaryThis research aimed to examine the effects of thyme, celery and salinomycin on ovarian sex hormones, reproductive traits and antioxidant status during the estrous cycle. Seventy-five mature Barki ewes aged 2–3 years with an average weight of 40 ± 1.5 kg were assigned randomly into five groups (15 head/group). Group 1 was kept as the control; groups 2 and 3 received 20 g/head/day thyme (T) and celery (C) as dried herbs, respectively. Group 4 (T×C) received 10 g thyme + 10 g celery/head/day, and group 5 was treated with salinomycin 1 g/head/day. Blood samples were collected during follicular and luteal phases of the estrous cycle. Thyme and celery and the mixture of T×C increased (P < 0.01) estradiol-17β (E2) during the follicular phase of the estrous cycle, while only the celery group showed a marked (P < 0.001) increase in progesterone (P4) during the luteal phase compared with the control. Salinomycin supplementation decreased (P < 0.05) E2 concentrations during the follicular and luteal phases of the estrous cycle. Supplementation with thyme and celery enhanced (P < 0.001) antioxidant capacity in the luteal phase compared with the follicular stage. The salinomycin group showed increased (P < 0.01) levels of reduced glutathione (GSH) and decreased malondialdehyde (MDA) levels compared with the control group throughout luteal phase. For the interaction between estrous phases and treatments, thyme, celery, and T×C supplementation revealed an increase (P < 0.05) in superoxide dismutase (SOD), GSH, and glutathione disulfide (GSSG) levels compared with the control group during the follicular and luteal phases. Thyme and celery supplementation improved the number of services per conception and fertilization from 1st and 2nd inseminations, respectively. In conclusion, the applied treatment had significant effects on reproductive performance and antioxidant status in ewes throughout the estrous cycle.

中文翻译：

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百里香、芹菜和盐霉素作为饲料添加剂的巴基母羊发情周期卵巢活性及抗氧化指标

摘要本研究旨在检查百里香、芹菜和盐霉素对发情周期期间卵巢性激素、生殖特征和抗氧化状态的影响。将 75 只 2-3 岁、平均体重 40 ± 1.5 kg 的成年 Barki 母羊随机分为 5 组（15 头/组）。第1组作为对照；第 2 组和第 3 组分别接受 20 克/头/天的百里香 (T) 和芹菜 (C) 作为干药草。第 4 组 (T×C) 接受 10 g 百里香 + 10 g 芹菜/头/天，第 5 组接受盐霉素 1 g/头/天。在动情周期的卵泡期和黄体期采集血样。百里香和芹菜以及T×C的混合物增加（磷< 0.01) estradiol-17β (E2) 在发情周期的卵泡期，而只有芹菜组表现出明显的 (磷< 0.001) 与对照相比，黄体期黄体酮 (P4) 增加。盐霉素补充剂减少（磷< 0.05) 发情周期卵泡期和黄体期的 E2 浓度。补充百里香和芹菜增强（磷< 0.001) 与卵泡期相比，黄体期的抗氧化能力。盐霉素组增加（磷< 0.01) 水平的还原型谷胱甘肽 (GSH) 和降低的丙二醛 (MDA) 水平与整个黄体期的对照组相比。对于发情期和治疗之间的相互作用，百里香、芹菜和 T×C 补充剂显示增加（磷< 0.05) 在卵泡期和黄体期的超氧化物歧化酶 (SOD)、GSH 和谷胱甘肽二硫化物 (GSSG) 水平与对照组相比。百里香和芹菜补充剂分别提高了第一次和第二次受精的每次受孕和受精的服务数量。总之，所应用的处理对整个发情周期的母羊的繁殖性能和抗氧化状态有显着影响。