Despite worldwide efforts to understand the transmission dynamics of Zika virus (ZIKV), scanty evaluation has been made on the vector competence of Aedes aegypti fed directly on viremic human and non-human primates (NHPs). We blood-fed Ae. aegypti from two districts in Rio de Janeiro on six ZIKV infected pregnant rhesus macaques at several time points, half of which were treated with Sofosbuvir (SOF). Mosquitoes were analyzed for vector competence after 3, 7 and 14 days of incubation. Although viremia extended up to eight days post monkey inoculation, only mosquitoes fed on the day of the peak of viremia, recorded on day two, became infected. The influence of SOF treatment could not be assessed because the drug was administered just after mosquito feeding on day two. The global infection, dissemination and transmission rates were quite low (4.09%, 1.91% and 0.54%, respectively); no mosquito was infected when viremia was below 1.26 × 10⁵ RNA copies/mL. In conclusion, Ae. aegypti vector competence for ZIKV from macaques is low, likely to be due to low viral load and the short duration of ZIKV viremia in primates suitable for infecting susceptible mosquitoes. If ZIKV infection in human and macaques behaves similarly, transmission of the Zika virus in nature is most strongly affected by vector density.

中文翻译：

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Viremic恒河猴的寨卡病毒的埃及伊蚊低效载体能力

尽管全世界都在努力了解寨卡病毒（ZIKV）的传播动态，但仍对直接饲喂有毒的人类和非人类灵长类动物（NHP）的埃及伊蚊的载体能力进行了评估。我们爱上了爱 埃及在几个时间点从里约热内卢两个地区的六只受ZIKV感染的猕猴进行了猕猴的感染，其中一半接受了Sofosbuvir（SOF）的治疗。在温育3、7和14天后，分析蚊子的载体能力。尽管病毒血症在猴子接种后可延长到八天，但只有在第二天记录的病毒血症高峰当天喂食的蚊子才被感染。无法评估SOF治疗的影响，因为该药物是在第二天蚊子喂食后立即服用的。全球感染，传播和传播率很低（分别为4.09％，1.91％和0.54％）；当病毒血症低于1.26×10 ⁵ RNA复制/ mL时，没有蚊子被感染。最后，Ae。埃及猕猴对ZIKV的矢量能力很低，这可能是由于低病毒载量和适合感染易感蚊子的灵长类动物ZIKV病毒血症的持续时间较短。如果人类和猕猴中的ZIKV感染行为相似，则自然中寨卡病毒的传播受载体密度的影响最大。