The incidence of foodborne diseases has continuingly increased over the years and resulted in public health problem globally. Enterohemorrhagic Escherichia coli O157:H7 (E. coli O157:H7) is a human pathogen that causes diarrhea and hemorrhagic colitis. E. coli O157:H7 can be found in various foods. It is important to detect this foodborne pathogen to provide safe food supply. A lot of methods, for example, culture and PCR‐based test, used to detect foodborne pathogens are laborious and time consuming. Hence, a variety of methods have been developed for rapid, simple and reliable detection of E. coli O157:H7 as it is required in many food analyses. Lateral flow immunoassay (LFIA) are advantageous over conventional detection methods in terms of their rapidity and simplicity for end user, especially the LFIA can be developed as the strip test for on‐site point‐of‐care test (POCT) products. Gold nanoparticles (AuNPs; colloid gold) are the most commonly used labels in the LFIA for the visual analysis, however, there are still several limitations that restrict their applications of traditional LFIA. Therefore, recent reports on improved LFIA for E. coli O157:H7 detection in foods are continuously reported. This review intends to provide these recent advances in improved LFIA methods for the detection of E. coli O157:H7 in foods.

中文翻译：

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食品中病原性大肠杆菌O157：H7的改进侧向流免疫测定的最新进展综述

多年来，食源性疾病的发病率持续增加，并在全球范围内导致了公共卫生问题。肠出血性大肠杆菌O157：H7（E. coli O157：H7）是引起腹泻和出血性结肠炎的人类病原体。大肠杆菌O157：H7可以在各种食物中找到。重要的是要检测这种食源性病原体，以提供安全的食物供应。用于检测食源性病原体的许多方法（例如，基于培养和PCR的检测）既费力又费时。因此，已经开发了多种方法来快速，简单和可靠地检测大肠杆菌。O157：H7，在许多食品分析中都需要。侧向流免疫测定法（LFIA）在快速性和最终用户简便性方面均优于常规检测方法，尤其是LFIA可以作为现场即时检验（POCT）产品的剥离测试开发。金纳米颗粒（AuNPs；胶体金）是LFIA中用于视觉分析的最常用标签，但是，仍然存在一些限制，限制了它们在传统LFIA中的应用。因此，不断有关于改善食品中大肠杆菌O157：H7检测的LFIA的最新报道。这篇综述旨在提供用于检测食品中大肠杆菌O157：H7的改良LFIA方法的最新进展。