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Nanogold‐based immunochromatographic strip test for rapid detection of clinical and environmental strains of Vibrio cholerae
Journal of Food Safety ( IF 1.9 ) Pub Date : 2020-11-23 , DOI: 10.1111/jfs.12874 Chalinan Pengsuk 1 , Pradit Wangman 2, 3 , Parin Chaivisuthangkura 2, 3 , Paisarn Sithigorngul 2, 3 , Siwaporn Longyant 2, 3
Journal of Food Safety ( IF 1.9 ) Pub Date : 2020-11-23 , DOI: 10.1111/jfs.12874 Chalinan Pengsuk 1 , Pradit Wangman 2, 3 , Parin Chaivisuthangkura 2, 3 , Paisarn Sithigorngul 2, 3 , Siwaporn Longyant 2, 3
Affiliation
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A nanogold‐based immunochromatographic strip test (VCG strip test) for the detection of all 70 isolates of Vibrio cholerae including O1, O139, and non‐O1/O139 (NVCs) was developed using two monoclonal antibodies (MAbs), namely VC‐63 and VC‐201, which bound specifically to 10 and 15 kDa proteins. Direct detection of V. cholerae in experimentally spiked fresh seafood samples such as shrimp, blood clam, mussel, and oyster could be achieved with sensitivities of 107 CFU/ml, which was similar to that of the dot blotting test using each MAb. The detection sensitivity could be improved to 103 or 10 or 1 CFU/ml of original bacterial content after preincubation of the sample in alkaline peptone water for 6, 12, and 24 hr consecutively. However, the detection sensitivities were also dependent on the content of other bacteria in the sample that might compete or inhibit the growth of V. cholerae during the preenrichment step. Due to its broad specificity, simplicity and rapid result generation, the VCG strip test can be used for the preliminary detection of V. cholerae at point of care for both environmental and clinical strains to assist appropriate decision or epidemiological surveillance of virulent V. cholerae strains (including NVC strains), which are ubiquitous in the environment and seafood.
中文翻译：
基于纳米金的免疫色谱带测试可快速检测霍乱弧菌的临床和环境菌株
使用两种单克隆抗体（VCb -63）开发了一种基于纳米金的免疫色谱剥离试验（VCG剥离试验），用于检测所有70株霍乱弧菌，包括O1，O139和非O1 / O139（NVC）。和VC-201，它们特异性结合10和15 kDa的蛋白质。直接检测的V。灵敏度为10 7 CFU / ml的新鲜海鲜样品（如虾，血蛤，贻贝和牡蛎）中的霍乱杆菌可以实现，这与使用每种单克隆抗体的斑点印迹法相似。检测灵敏度可以提高到10 3在碱性蛋白ept水中将样品预孵育连续6、12和24小时后，原始细菌含量为10或1 CFU / ml。但是，检测灵敏度还取决于样品中可能竞争或抑制V增长的其他细菌的含量。富集步骤中的霍乱。由于其广泛的特异性，简便性和快速的结果生成能力，因此可以将VCG试纸条用于V的初步检测。霍乱弧菌在医疗点的环境和临床株，以协助适当的决定或有毒的流行病学监测V。霍乱 菌株（包括NVC菌株），在环境和海产品中普遍存在。
更新日期：2020-11-23
中文翻译：
基于纳米金的免疫色谱带测试可快速检测霍乱弧菌的临床和环境菌株
使用两种单克隆抗体（VCb -63）开发了一种基于纳米金的免疫色谱剥离试验（VCG剥离试验），用于检测所有70株霍乱弧菌，包括O1，O139和非O1 / O139（NVC）。和VC-201，它们特异性结合10和15 kDa的蛋白质。直接检测的V。灵敏度为10 7 CFU / ml的新鲜海鲜样品（如虾，血蛤，贻贝和牡蛎）中的霍乱杆菌可以实现，这与使用每种单克隆抗体的斑点印迹法相似。检测灵敏度可以提高到10 3在碱性蛋白ept水中将样品预孵育连续6、12和24小时后，原始细菌含量为10或1 CFU / ml。但是，检测灵敏度还取决于样品中可能竞争或抑制V增长的其他细菌的含量。富集步骤中的霍乱。由于其广泛的特异性，简便性和快速的结果生成能力，因此可以将VCG试纸条用于V的初步检测。霍乱弧菌在医疗点的环境和临床株，以协助适当的决定或有毒的流行病学监测V。霍乱 菌株（包括NVC菌株），在环境和海产品中普遍存在。
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