The control of nitrogen (N) flows via nitrates leaching and nitrous oxide emission is crucial for a safe environment. Some plants have shown abilities to suppress soil nitrification by releasing biological inhibitors, such as sorgoleone in sorghum. We hypothesized that the N uptake capacity of plants partly influences soil nitrification, and so do the intensity of the arbuscular mycorrhizal fungi (AMF)-sorghum symbiosis. Therefore, this study clarified the relationships between sorghum biological nitrification inhibition levels and AMF and fungi abundances in rhizosphere soils and sorghum roots by qPCR. The varieties 296B, IS32234, and IS20205, which release low (L-sorg), medium (M-sorg), and high (H-sorg) sorgoleone, respectively, were grown in φ 12 cm × 1 m pipes. Including a control without plant, they received 0 and 120 kg N ha⁻¹ as ammonium sulfate solution. Samples were collected from 0 to 10, 10–30, and 30–80 cm soil layers. AMF and fungi populations at 31 days were significantly higher in H-sorg followed by M-sorg soils. The application of 120 kg N ha⁻¹ (70 days) substantially increased AMF’s abundance in the 0–10 cm horizon. This result reveals that N-rich environments favor AMF expression. The AMF increase was more marked in the H-sorg and M-sorg soils, indicating that the levels of sorgoleone released may also influence the AMF symbiosis. Although H-sorg harbored more endophytic fungi, we did not observe any difference in root AMF colonization. The higher sorgoleone varieties showed higher plant biomass and N amounts, reflecting better N absorption and assimilation. The study reveals that sorgoleone, which confers sorghum its nitrification inhibition ability, also strengthens the sorghum-AMF symbiosis, N uptake and reduces the ammonia pool subjected to nitrification, and possibly the loss of nitrogen.

通过硝酸盐浸出和一氧化二氮排放来控制氮（N）流量对于安全环境至关重要。一些植物显示出通过释放生物抑制剂（例如高粱中的高粱油酮）来抑制土壤硝化的能力。我们假设植物的氮吸收能力会部分影响土壤硝化作用，丛枝菌根真菌（AMF）-高粱共生强度也会受到影响。因此，本研究通过qPCR阐明了根际土壤和高粱根中高粱生物硝化抑制水平与AMF和真菌丰度之间的关系。在φ12 cm×1 m的管道中种植了分别释放低（L-sorg），中（M-sorg）和高（H-sorg）高粱油酮的品种296B，IS32234和IS20205。包括一个没有植物的对照，他们获得了0和120千克N ha^-1为硫酸铵溶液。从0到10、10–30和30–80厘米的土壤层中收集样品。H-sorg土壤中31天的AMF和真菌种群显着较高，其次是M-sorg土壤。120 kg N ha ^-1的施用（70天）在0-10厘米水平范围内大大增加了AMF的丰度。该结果表明，富氮环境有利于AMF表达。H-org和M-sorg土壤中AMF的增加更为明显，这表明释放的高粱油酮水平也可能影响AMF共生。尽管H-sorg含有更多的内生真菌，但我们在根AMF的定殖方面没有发现任何差异。较高的高粱油菜品种显示出较高的植物生物量和氮含量，反映了较好的氮吸收和同化作用。研究表明，赋予高粱硝化抑制能力的高粱油酮还增强了高粱与AMF的共生，吸收氮并减少了硝化作用下氨气的积累，并可能减少了氮的流失。