Biofilms characterize sessile form that allows bacterial maintenance under hostile conditions. Salmonella represents an important foodborne zoonotic agent, capable of forming biofilms on diverse surfaces. The emergence of Salmonella Minnesota in the Brazilian poultry production expresses the need for more specific knowledge related to its maintenance in the environment and consequent food contamination, as well as control measures. Our approach combines the analysis of genetic determinants linked to biofilm formation and the phenotypic study of biomass on different surfaces, together with the determination of the effect of chemical agents on the control of sessile structure. It also evaluates the genetic similarity profile of 29 S. Minnesota strains isolated from food and environmental samples in two full-cycle poultry industries from 2009 to 2014. Genetic analysis showed low heterogeneity with the identification of six clonal groups, three clusters with homology greater than 80% and seven distinct genotypes. It was compatible with the presence of important genes in biofilm formation, with 28/29 (97.0%) presenting adrA and csgD and 27 (93.0%) presenting the luxS gene. Allied to this, 19/29 of the strains (66.0%) presented biomass that varied the intensity in weak (9/19 – 47.0%), moderate (6/19 – 32.0%) and strong (4/19 – 21.0%), with characteristic ultrastructure of a mature biofilm. Sodium hypochlorite showed greater efficiency in the control of sessile cells, even after incubation process. The study shows the presence of biofilm-producing S. Minnesota characterizing the problem of persistence in broiler slaughterhouses and indicates as a control strategy the use of sodium hypochlorite in an appropriate manner.

生物膜表征无梗形式，其允许在不利条件下维持细菌。沙门氏菌是一种重要的食源性人畜共患病药物，能够在多种表面上形成生物膜。出现沙门氏菌明尼苏达州在巴西家禽生产表达了有关其在环境和随之而来的食品污染的维护，以及控制措施的具体知识的需要。我们的方法结合了与生物膜形成相关的遗传决定因素分析和不同表面生物量的表型研究，以及化学药剂对无柄结构控制作用的确定。它还评估了29 S的遗传相似性。从2009年至2014年从两个全周期家禽业的食品和环境样品中分离出的明尼苏达菌株。遗传分析显示，异源性低，鉴定了六个克隆组，三个同源性大于80％的簇和七个不同的基因型。它与生物膜形成中重要基因的存在相容，其中28/29（97.0％）代表adrA和csgD，27（93.0％）代表luxS。基因。与此相关的是，有19/29个菌株（66.0％）呈现出的生物量在弱（9/19 – 47.0％），中度（6/19 – 32.0％）和强（4/19 – 21.0％）时变化，具有成熟生物膜的特有超微结构。次氯酸钠即使在温育过程中也显示出更高的控制无柄细胞的效率。研究表明存在生物膜产生S.明尼苏达表征持久性的问题在肉鸡屠宰场和作为控制策略表示以适当的方式使用的次氯酸钠。