Diazinon is a common organophosphate pesticide widely used to control parasitic infections in agriculture. Excessive use of diazinon can have adverse effects on the environment and aquatic animal health. In the present study, the toxic effects of diazinon on the histology, antioxidant, innate immune and intestinal microbiota community composition of crucian carp (Carassius auratus gibelio) were investigated. The results showed that diazinon at the tested concentration (300 μg/L) induced gill and liver histopathological damages. Hepatic total superoxide dismutase (T-SOD), catalase (CAT), and glutathione S-transferase (GST) activities significantly decreased (P<0.05) by 32.47%, 65.33% and 37.34%, respectively. However, the liver tissue malondialdehyde (MDA) content significantly (P<0.05) increased by 138.83%. The 300 μg/L diazinon significantly (P<0.05) downregulated the gene expression of TLR4, MyD88, NF-kB p100 and IL-8 but had no significant effect TNF-α (P=0.8239). In addition, the results demonstrated that diazinon exposure could affect the intestinal microbiota composition and diversity. Taken together, the results of this study indicated that diazinon exposure can cause damage to crucian carp, induce histopathological damage in gill and liver tissues, oxidative stress in the liver, and innate immune disorders and alter intestinal microbiota composition and diversity.

二嗪农是一种常见的有机磷农药，广泛用于控制农业中的寄生虫感染。过量使用二嗪农会对环境和水生动物健康产生不利影响。在本研究中，二嗪磷对组织学，抗氧化剂，先天免疫和鲫鱼的肠道微生物群落的组合物（毒性作用银鲫）进行了调查。结果表明，浓度为300μg/ L的二嗪农会引起g和肝脏组织病理学损害。肝总超氧化物歧化酶（T-SOD），过氧化氢酶（CAT）和谷胱甘肽S-转移酶（GST）活性分别显着降低（P <0.05），分别降低32.47％，65.33％和37.34％。但是，肝脏组织中丙二醛（MDA）含量显着（P <0.05）增加了138.83％。在300微克/升二嗪农显著（P <0.05）下调TLR4，MyD88的，NF-kB的P100和IL-8基因表达，但没有显著影响TNF-α（P = 0.8239）。此外，结果表明二嗪农暴露可能会影响肠道菌群的组成和多样性。两者合计，这项研究的结果表明，暴露于二嗪农会造成cru鱼的损害，在g和肝组织中引起组织病理学损害，在肝脏中产生氧化应激，以及先天性免疫疾病并改变肠道菌群的组成和多样性。