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A semi-dry chemistry hydrogel-based smart biosensing platform for on-site detection of metal ions
Lab on a Chip ( IF 6.1 ) Pub Date : 2020-11-24 , DOI: 10.1039/d0lc00855a Xiaoxia Mao 1 , Dongsheng Mao , Juanjuan Jiang , Benyue Su , Guifang Chen , Xiaoli Zhu
Lab on a Chip ( IF 6.1 ) Pub Date : 2020-11-24 , DOI: 10.1039/d0lc00855a Xiaoxia Mao 1 , Dongsheng Mao , Juanjuan Jiang , Benyue Su , Guifang Chen , Xiaoli Zhu
Affiliation
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Balancing operability and performance has long been a focus of research in bioanalysis and biosensing. In this work, between the traditional wet chemistry and dry chemistry, we develop a semi-dry smart biosensing platform with favourable operability and performance for metal ions detection. This platform is based on the integration of a stimuli-responsive hydrogel with intelligent image recognition. The hydrogel consists of agarose as a matrix and well-designed fluorescent DNA probes as response elements. Target metal ions in a test sample can diffuse into the hydrogel and activate the DNA probes, outputting fluorescence signals for intelligent imaging. In this way, sensitive and convenient detection of metal ions such as potassium ions (K+) and mercury ions (Hg2+) can be achieved without the assistance of huge instruments and professional workers. The detection limits for K+ and Hg2+ are 0.34 mM and 5.6 nM, respectively. Detection of ions in serum and lake water is also available. Moreover, the hydrogel-based biosensing platform exhibits favorable selectivity, anti-degradation ability, and long-term stability. High-throughput testing can be also achieved by punching multiple test microwells in a single piece of hydrogel. The concept and successful practice of a semi-dry chemistry-based strategy make up for the shortcomings of wet chemistry and dry chemistry, and provide a promising approach for on-site testing.
中文翻译：
基于半干化学水凝胶的智能生物传感平台，用于金属离子的现场检测
长期以来，平衡可操作性和性能一直是生物分析和生物传感研究的重点。在这项工作中，我们在传统的湿化学和干化学之间，开发了一种具有良好的可操作性和性能的半干式智能生物传感平台，可用于金属离子检测。该平台基于刺激响应水凝胶与智能图像识别的集成。水凝胶由琼脂糖为基质，精心设计的荧光DNA探针为响应元件。测试样品中的目标金属离子可以扩散到水凝胶中并激活DNA探针，输出荧光信号进行智能成像。这样，可以灵敏，方便地检测金属离子，例如钾离子（K +）和汞离子（Hg 2+）无需大型仪器和专业工作者的帮助即可实现。K +和Hg 2+的检出限分别为0.34 mM和5.6 nM。还可以检测血清和湖水中的离子。此外，基于水凝胶的生物传感平台具有良好的选择性，抗降解能力和长期稳定性。高通量测试也可以通过在单片水凝胶中冲孔多个测试微孔来实现。基于半干化学的策略的概念和成功实践弥补了湿化学和干化学的缺点，并为现场测试提供了一种有希望的方法。
更新日期：2020-12-10
中文翻译：
基于半干化学水凝胶的智能生物传感平台，用于金属离子的现场检测
长期以来，平衡可操作性和性能一直是生物分析和生物传感研究的重点。在这项工作中，我们在传统的湿化学和干化学之间，开发了一种具有良好的可操作性和性能的半干式智能生物传感平台，可用于金属离子检测。该平台基于刺激响应水凝胶与智能图像识别的集成。水凝胶由琼脂糖为基质，精心设计的荧光DNA探针为响应元件。测试样品中的目标金属离子可以扩散到水凝胶中并激活DNA探针，输出荧光信号进行智能成像。这样，可以灵敏，方便地检测金属离子，例如钾离子（K +）和汞离子（Hg 2+）无需大型仪器和专业工作者的帮助即可实现。K +和Hg 2+的检出限分别为0.34 mM和5.6 nM。还可以检测血清和湖水中的离子。此外，基于水凝胶的生物传感平台具有良好的选择性，抗降解能力和长期稳定性。高通量测试也可以通过在单片水凝胶中冲孔多个测试微孔来实现。基于半干化学的策略的概念和成功实践弥补了湿化学和干化学的缺点，并为现场测试提供了一种有希望的方法。
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