Since early diagnosis of sepsis may assist clinicians in initiating timely, effective, and prognosis-improving antibiotic therapy, we developed an integrated microfluidic chip (IMC) for rapid isolation of both Gram-positive and Gram-negative bacteria from blood. The device comprised a membrane-based filtration module (90 min operating time), a bacteria-capturing module using a micro-mixer containing magnetic beads coated with “flexible neck” regions of mannose-binding lectin proteins for bacteria capture (20 min), and a miniature polymerase chain reaction (PCR) module for bacteria identification (90 min via TaqMan® probe technology). The filter separated all white blood cells and 99.5% of red blood cells from bacteria, which were captured at rates approaching 85%. The PCR assay's limit of detection was 5 colony-forming units (CFU) per reaction, and the entire process was completed in only 4 h. Since this is far less than that for culture-based approaches, this IMC may serve as a promising device for detection of sepsis.

中文翻译：

---

早期检测败血症诱导细菌的集成微流控系统

由于脓毒症的早期诊断可以帮助临床医生及时，有效和改善预后，因此我们开发了一种集成的微流控芯片（IMC），可从血液中快速分离革兰氏阳性和阴性细菌。该设备包括一个基于膜的过滤模块（运行时间为90分钟），一个使用微型混合器的细菌捕获模块，该混合器包含涂有甘露糖结合凝集素蛋白“柔性颈部”区域的磁珠，用于细菌捕获（20分钟），以及用于细菌鉴定的微型聚合酶链反应（PCR）模块（通过TaqMan®探针技术）。过滤器从细菌中分离出所有白细胞和99.5％的红细胞，这些细菌的捕获率接近85％。PCR检测的检测极限是每个反应5个菌落形成单位（CFU），整个过程仅需4小时即可完成。由于这远远少于基于培养的方法，因此该IMC可以作为检测脓毒症的有前途的设备。