Osmium nanozyme as peroxidase mimic with high performance and negligible interference of O2,Journal of Materials Chemistry A

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Osmium nanozyme as peroxidase mimic with high performance and negligible interference of O2
Journal of Materials Chemistry A ( IF 10.7 ) Pub Date : 2020-11-14 , DOI: 10.1039/d0ta09247a
Shaobin He _{1,

2,

3,

4,

5} , Liu Yang _{1,

2,

3,

4,

5} , Paramasivam Balasubramanian _{1,

2,

3,

4,

5} , Shujun Li _{1,

2,

3,

4,

5} , Huaping Peng _{1,

2,

3,

4,

5} , Ye Kuang _{1,

2,

3,

4,

5} , Haohua Deng _{1,

2,

3,

4,

5} , Wei Chen _{1,

2,

3,

4,

5}

Affiliation

Ongoing efforts have contributed to the exploration of peroxidase-like nanozymes while preserving their benefits in bioassays. Unfortunately, many peroxidase-like nanozymes still exhibit a low catalytic activity accompanied by oxidase-like activity with interferences of O₂ and color which limit their applicability. Herein, we carried out a “green” approach to synthesize citrate-coated osmium nanozyme with a high peroxidase-like performance. Comprehensive experiments were validated in which the Os nanozyme could achieve excellent steady-state kinetic parameters and a high specific activity (393 000 Unit per g), which was better than those of most reported nanozymes. More significantly, the Os nanozyme possesses a higher peroxidase-like activity and negligible oxidase-like activity compared with those of common noble metal-based nanozymes (Pt and Au), freeing the corresponding assay system from the interference of O₂ and color. In addition, the Os nanozyme presents robustness against complex environments and some metal ions that usually act as noble metal-based nanozymes. As a proof-of-concept, the Os nanozyme has been successfully employed to fabricate colorimetric assays for glucose and pyruvic acid detections with the associated color gradients. These findings collectively prove that the Os nanozyme could be a novel and promising peroxidase mimic candidate for bioassays and beyond.

中文翻译：

nano纳米酶作为过氧化物酶，具有高性能且对O2的干扰可忽略不计

正在进行的努力为过氧化物酶样纳米酶的探索做出了贡献，同时保留了它们在生物测定中的优势。不幸的是，许多过氧化物酶样纳米酶仍然表现出较低的催化活性，同时伴随着氧化酶样活性和O _2的干扰。和颜色限制了它们的适用性。在这里，我们进行了一种“绿色”方法，以合成具有高过氧化物酶样性能的柠檬酸盐包覆的nano纳米酶。验证了全面的实验，其中Os纳米酶可以实现出色的稳态动力学参数和较高的比活度（393 000单位/克），这比大多数报道的纳米酶要好。更重要的是，与普通的基于贵金属的纳米酶（Pt和Au）相比，Os纳米酶具有更高的过氧化物酶样活性和可忽略的氧化酶样活性，从而使相应的测定系统摆脱了O ₂的干扰。和颜色。另外，Os纳米酶对复杂的环境和一些通常作为贵金属基纳米酶起作用的金属离子具有很强的抵抗力。作为概念证明，Os纳米酶已成功地用于制造葡萄糖和丙酮酸检测的色度测定法以及相关的颜色梯度。这些发现共同证明，Os纳米酶可能是用于生物测定及其他领域的新型且有希望的过氧化物酶模拟物候选物。

更新日期：2020-11-25

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