Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Effect of proteins on the oxidase-like activity of CeO2 nanozymes for immunoassays
Analyst ( IF 3.6 ) Pub Date : 2020-11-03 , DOI: 10.1039/d0an01755h Zi-Jian Chen 1, 2, 3, 4, 5 , Zhicheng Huang 5, 6, 7, 8, 9 , Song Huang 4, 10, 11 , Jin-Lin Zhao 4, 10, 11 , Yuanming Sun 1, 2, 3, 4 , Zhen-Lin Xu 1, 2, 3, 4 , Juewen Liu 5, 6, 7, 8, 9
Analyst ( IF 3.6 ) Pub Date : 2020-11-03 , DOI: 10.1039/d0an01755h Zi-Jian Chen 1, 2, 3, 4, 5 , Zhicheng Huang 5, 6, 7, 8, 9 , Song Huang 4, 10, 11 , Jin-Lin Zhao 4, 10, 11 , Yuanming Sun 1, 2, 3, 4 , Zhen-Lin Xu 1, 2, 3, 4 , Juewen Liu 5, 6, 7, 8, 9
Affiliation
|
Having the benefits of low cost, excellent stability and tolerance to extreme conditions, nanozymes are a potential alternative of horseradish peroxidase (HRP) or other enzymes for bioanalytical chemistry, especially immunoassays. CeO2 nanoparticles have oxidase-mimicking activity and can avoid the use of unstable H2O2. For robust assays, the effect of proteins on the activity of CeO2 needs to be carefully studied. Herein, we studied the adsorption and desorption of bovine serum albumin (BSA) from CeO2. The CeO2 nanoparticles exhibited a higher protein adsorption capacity compared to the other tested metal oxide nanoparticles. Although the oxidase-like activity of CeO2 was inhibited by BSA, low concentrations of phosphate and fluoride ions boosted the activity of protein-capped CeO2. CeO2 was still active under strong acidic conditions and at high temperature, while HRP lost its activity. For immunoassay development, we covered CeO2 with an amine-modified silane for covalent conjugation to antibodies. A one-step indirect competitive ELISA for fenitrothion was developed, and an IC50 value of 35.6 ng mL−1 and a limit of detection of 2.1 ng mL−1 were obtained.
中文翻译：
蛋白质对免疫分析中CeO2纳米酶氧化酶样活性的影响
纳米酶具有低成本，出色的稳定性和对极端条件的耐受性的优点，是辣根过氧化物酶（HRP）或其他酶的潜在替代品，可用于生物分析化学，尤其是免疫分析。CeO 2纳米颗粒具有模仿氧化酶的活性，可以避免使用不稳定的H 2 O 2。对于可靠的测定，需要仔细研究蛋白质对CeO 2活性的影响。在本文中，我们研究了牛血清白蛋白（BSA）从CeO 2的吸附和解吸。与其他测试的金属氧化物纳米颗粒相比，CeO 2纳米颗粒表现出更高的蛋白质吸附能力。尽管CeO 2具有类似氧化酶的活性被BSA抑制，低浓度的磷酸根和氟离子增强了蛋白质封端的CeO 2的活性。CeO 2在强酸性条件下和高温下仍具有活性，而HRP失去活性。对于免疫测定的开发，我们用胺修饰的硅烷覆盖了CeO 2以与抗体共价偶联。开发了一种用于杀nitro硫磷的单步间接竞争ELISA，获得的IC 50值为35.6 ng mL -1和检测限为2.1 ng mL -1。
更新日期：2020-11-25
中文翻译：
蛋白质对免疫分析中CeO2纳米酶氧化酶样活性的影响
纳米酶具有低成本，出色的稳定性和对极端条件的耐受性的优点，是辣根过氧化物酶（HRP）或其他酶的潜在替代品，可用于生物分析化学，尤其是免疫分析。CeO 2纳米颗粒具有模仿氧化酶的活性，可以避免使用不稳定的H 2 O 2。对于可靠的测定，需要仔细研究蛋白质对CeO 2活性的影响。在本文中，我们研究了牛血清白蛋白（BSA）从CeO 2的吸附和解吸。与其他测试的金属氧化物纳米颗粒相比，CeO 2纳米颗粒表现出更高的蛋白质吸附能力。尽管CeO 2具有类似氧化酶的活性被BSA抑制，低浓度的磷酸根和氟离子增强了蛋白质封端的CeO 2的活性。CeO 2在强酸性条件下和高温下仍具有活性，而HRP失去活性。对于免疫测定的开发，我们用胺修饰的硅烷覆盖了CeO 2以与抗体共价偶联。开发了一种用于杀nitro硫磷的单步间接竞争ELISA，获得的IC 50值为35.6 ng mL -1和检测限为2.1 ng mL -1。
京公网安备 11010802027423号