Alkaline phosphatase (ALP) is an essential hydrolase and widely distributed in living organisms. It plays important roles in various physiological and pathological processes. Herein, a turn-on near-infrared (NIR) fluorescent probe (DXMP) was developed for sensitive detection of ALP activity both in vitro and in vivo based on the intramolecular charge transfer (ICT) mechanism. Upon incubation with ALP, DXMP exhibited a strong fluorescence increment at 640 nm, which was attributed to the fact that ALP-catalyzed cleavage of the phosphate group in DXMP induced the transformation of DXMP into DXM-OH. The probe exhibited prominent features including outstanding selectivity, high sensitivity, and excellent biocompatibility. More importantly, it has been successfully used to detect and image endogenous ALP in living cells and zebrafish.

中文翻译：

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开启式近红外荧光探针，用于观察活细胞和斑马鱼中的内源性碱性磷酸酶活性

碱性磷酸酶（ALP）是必不可少的水解酶，广泛分布于生物体中。它在各种生理和病理过程中起重要作用。在此，导通近红外（NIR）荧光探针（DXMP）中的溶液两者的ALP活性的灵敏检测开发体外和体内基于分子内电荷转移（ICT）机制。与ALP一起孵育后，DXMP在640 nm处显示出很强的荧光增量，这归因于ALP催化的DXMP中磷酸基团的裂解诱导DXMP转变为DXM-OH。该探针具有突出的特征，包括出色的选择性，高灵敏度和出色的生物相容性。更重要的是，它已成功用于检测活细胞和斑马鱼中的内源性ALP并对其成像。