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Enhanced Methane Recovery from Waste-Activated Sludge by Alginate-Degrading Consortia: The Overlooked Role of Alginate in Extracellular Polymeric Substances
Environmental Science & Technology Letters ( IF 8.9 ) Pub Date : 2020-11-24 , DOI: 10.1021/acs.estlett.0c00784 Fang Zhang 1 , Ding-Kang Qian 1 , Zi-Qian Geng 1 , Kun Dai 1 , Wei Zhang 1 , Mark C. M. van Loosdrecht 2 , Raymond Jianxiong Zeng 1
Environmental Science & Technology Letters ( IF 8.9 ) Pub Date : 2020-11-24 , DOI: 10.1021/acs.estlett.0c00784 Fang Zhang 1 , Ding-Kang Qian 1 , Zi-Qian Geng 1 , Kun Dai 1 , Wei Zhang 1 , Mark C. M. van Loosdrecht 2 , Raymond Jianxiong Zeng 1
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The hydrolysis of extracellular polymeric substances (EPS) in waste-activated sludge (WAS) is considered as the rate-limiting step in anaerobic digestion. Uronic acids such as alginate are one of main polysaccharide components in EPS; however, their roles on WAS conversion are overlooked until now. Previously, we described alginate-degrading consortia (ADC) that have high activity for alginate conversion. In this work, ADC was studied for polysaccharide hydrolysis and methane production from WAS for the first time, which increased the methane production by 115%–185%. Dosing ADC also increased the values of biological methane potential from 131 to 172 mL/gVSS. An alginate-like exopolysaccharide was extracted from WAS, and the content was 65 mg/g-VSS. Then, the molecular weight profiles at UV254nm showed that disaccharides were the final hydrolysates of alginate by ADC enzyme. Extracted EPS could be utilized by ADC for methane production with acetate as the main intermediate. The mechanism was proposed that ADC played a key role in WAS conversion. These results indicated that alginate in EPS shall not be overlooked, which offers a new microbial method to enhance methane recovery from WAS. The microbial changes in ADC for the stability of WAS digestion should be investigated in the future.
中文翻译：
藻酸盐降解联合体从废物活化污泥中提高甲烷回收率：藻酸盐在细胞外聚合物中的被忽略作用
废物活化污泥（WAS）中细胞外聚合物（EPS）的水解被认为是厌氧消化中的限速步骤。尿酸（例如藻酸盐）是EPS中的主要多糖成分之一。但是，到目前为止，它们在WAS转换中的作用仍被忽略。以前，我们描述了藻酸盐降解协会（ADC），该协会对藻酸盐转化具有很高的活性。在这项工作中，首次对ADC进行了WAS多糖水解和甲烷生产的研究，这使甲烷产量提高了115％–185％。定量ADC也将生物甲烷气的值从131增加到172 mL / gVSS。从WAS中提取出藻酸盐样胞外多糖，含量为65mg / g-VSS。然后，在UV 254nm处的分子量分布结果表明，二糖是ADC酶水解藻酸盐的最终产物。ADC可以将提取的EPS用于以甲烷为主要中间体的甲烷生产。提出了ADC在WAS转换中起关键作用的机制。这些结果表明EPS中的藻酸盐不可忽视，这为提高WAS的甲烷回收率提供了一种新的微生物方法。将来应研究ADC对WAS消化稳定性的微生物变化。
更新日期：2020-11-25
中文翻译：
藻酸盐降解联合体从废物活化污泥中提高甲烷回收率：藻酸盐在细胞外聚合物中的被忽略作用
废物活化污泥（WAS）中细胞外聚合物（EPS）的水解被认为是厌氧消化中的限速步骤。尿酸（例如藻酸盐）是EPS中的主要多糖成分之一。但是，到目前为止，它们在WAS转换中的作用仍被忽略。以前，我们描述了藻酸盐降解协会（ADC），该协会对藻酸盐转化具有很高的活性。在这项工作中，首次对ADC进行了WAS多糖水解和甲烷生产的研究，这使甲烷产量提高了115％–185％。定量ADC也将生物甲烷气的值从131增加到172 mL / gVSS。从WAS中提取出藻酸盐样胞外多糖，含量为65mg / g-VSS。然后，在UV 254nm处的分子量分布结果表明，二糖是ADC酶水解藻酸盐的最终产物。ADC可以将提取的EPS用于以甲烷为主要中间体的甲烷生产。提出了ADC在WAS转换中起关键作用的机制。这些结果表明EPS中的藻酸盐不可忽视，这为提高WAS的甲烷回收率提供了一种新的微生物方法。将来应研究ADC对WAS消化稳定性的微生物变化。
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