The data situation of laser-induced damage measurements after multiple-pulse irradiation in the ns-time regime is limited. Since the laser safety standard is based on damage experiments, it is crucial to determine damage thresholds. For a better understanding of the underlying damage mechanism after repetitive irradiation, we generate damage thresholds for pulse sequences up to N = 20 000 with 1.8 ns-pulses using a square-core fiber and a pulsed Nd:YAG laser. Porcine retinal pigment epithelial layers were used as tissue samples, irradiated with six pulse sequences and evaluated for damage by fluorescence microscopy. The damage thresholds decreased from 31.16 µJ for N = 1 to 11.56 µJ for N = 20 000. The reduction indicates photo-chemical damage mechanisms after reaching a critical energy dose.

中文翻译：

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ns 时间范围内视网膜外植体的多脉冲损伤阈值


纳秒级多脉冲辐照后激光损伤测量的数据情况有限。由于激光安全标准基于损伤实验，因此确定损伤阈值至关重要。为了更好地了解重复照射后的潜在损伤机制，我们使用方芯光纤和脉冲 Nd:YAG 激光器，以 1.8 ns 脉冲生成高达 N = 20 000 的脉冲序列的损伤阈值。使用猪视网膜色素上皮层作为组织样本，用六个脉冲序列进行照射，并通过荧光显微镜评估损伤情况。损伤阈值从 N = 1 时的 31.16 µJ 降至 N = 20 000 时的 11.56 µJ。这种降低表明达到临界能量剂量后的光化学损伤机制。