Cell Adhesion and Migration Behaviors on Patterned Thermoresponsive Microgel Stripes,ACS Applied Bio Materials

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Cell Adhesion and Migration Behaviors on Patterned Thermoresponsive Microgel Stripes
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2020-11-23 , DOI: 10.1021/acsabm.0c00914
Yongqing Xia ₁ , Dachao Tang ₁ , Zhujun Zeng ₁ , Xiaojuan Wang ₂ , Shengjie Wang ₁

Affiliation

Cell adhesion and migration behaviors are influenced by their surrounding environments. In this work, patterned poly(N-isopropylacrylamide-styrene) (pNIPAAmSt) microgel stripes, which can provide a more complex environment, were fabricated on polyethyleneimine (PEI)-precoated glass substrate, and their effects on cell adhesion and migration behavior were investigated. The results showed that patterned cell layers can be formed on the space regions either by selective adhesion of the cells or by detaching the attached cells from the thermoresponsive microgel stripes via mild temperature stimuli. The migration behavior of the cells was affected by both the cell types and the width of the microgel stripes. The wider the microgel stripe, the harder it is for the cells to migrate, and the longer the patterned cell layers can be conserved. It is worth noting that the cell–cell interaction in the cocultured system plays a key role in the cell migration process. This work may offer a platform to study the adhesion and migration behavior of mono/cocultured cells in a more controllable system.

中文翻译：

图案化热响应微凝胶条纹上的细胞粘附和迁移行为

细胞粘附和迁移行为受其周围环境的影响。在这项工作中，图案化的聚（N-异丙基丙烯酰胺-苯乙烯) (pNIPAAmSt) 微凝胶条带可提供更复杂的环境，在聚乙烯亚胺 (PEI) 预涂玻璃基板上制备，并研究了它们对细胞粘附和迁移行为的影响。结果表明，通过细胞的选择性粘附或通过温和的温度刺激将附着的细胞从热响应微凝胶条带上分离，可以在空间区域上形成图案化的细胞层。细胞的迁移行为受细胞类型和微凝胶条纹宽度的影响。微凝胶条纹越宽，细胞迁移就越困难，图案化的细胞层可以保存的时间越长。值得注意的是，共培养系统中的细胞间相互作用在细胞迁移过程中起着关键作用。

更新日期：2020-12-21

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