Abstract A total of 720 one-day-old male broiler chickens were randomly allotted into nine treatments to evaluate the effects of different levels of phytase (Phy, 0.0, 500, and 1000 FTU/kg) and benzoic acid (BA, 0.0, 0.5, and 1.0 g/kg) supplementation on performance, nutrients digestibility, tibia mineralisation, intestinal morphology and serum traits in broiler chickens fed nutrient deficient diets (NC). The experiment was done in a 3*3 factorial arrangements with a completely randomised design until 42 days of age. Body weight (BW), body weight gain (BWG), and feed conversion ratio (FCR) improved when Phy (at both levels) was added to NC diets (p ≤ .05). Phy at 1X and 2X increased serum P, decreased serum Ca and litter moisture content (1000 FTU/kg) at d 28 (p ≤ .05), Furthermore, Phy supplementation increased tibia length and ash content as well as apparent ileal digestibility of P and ileal villus height (VH) (p ≤ .05). Addition of BA at 0.5 g/kg to the diets increased litter moisture, serum Ca content, jejunal villus height and ileal villus width (VW) (p ≤ .05). However, the combination of Phy and BA supplementation decreased apparent ileal of Ca digestibility (at 2X), and increased VH/CD ratio of the jejunum (at 1X). In conclusion, Phy was effective in improving performance traits, bone mineralisation, apparent ileal nutrients digestibility, and intestinal morphology. BA had no impact on performance traits, bone mineralisation, and ileal nutrients digestibility but at recommended level (0.5 g/kg) increased VH and VW in the ileum and jejunum. Highlights Supplementation of nutrient deficient diet with phytase significantly improved body weight and body weightgain in male broiler chickens. Phytase supplementation increased serum phosphorus content and decreased serum calcium and litter moisture contents. Phytase supplementation increased tibia length and ash content, apparent ileal digestibility of phosphorus and ileal villus height. Addition of benzoic acid to nutrient deficient diet increased litter moisture, serum calcium content, jejunal villus height and ileal villus width. Combination of Phytase and benzoic acid supplementations decreased apparent ileal digestibility of calcium, crypt depth of the ileum and increased villus width and villus height/crypt depth ratio of the jejunum and ileum, respectively.

中文翻译：

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评估添加植酸酶和苯甲酸对雄性肉鸡性能、营养物质消化率、胫骨矿化、肠道形态和血清性状的相互作用影响

摘要 将 720 只 1 日龄雄性肉鸡随机分配到 9 个处理组，评价不同水平植酸酶（Phy，0.0、500 和 1000 FTU/kg）和苯甲酸（BA，0.0、0.5和 1.0 g/kg) 补充剂对饲喂营养缺乏日粮 (NC) 的肉鸡的性能、营养物质消化率、胫骨矿化、肠道形态和血清特征的影响。该实验以 3*3 因子安排进行，采用完全随机设计，直至 42 日龄。将 Phy（两种水平）添加到 NC 日粮后，体重 (BW)、体重增加 (BWG) 和饲料转化率 (FCR) 得到改善 (p ≤ .05)。1X 和 2X 的 Phy 在第 28 天 (p ≤ .05) 增加血清 P，降低血清 Ca 和垫料水分含量 (1000 FTU/kg)，此外，Phy 补充剂增加了胫骨长度和灰分含量以及 P 的表观回肠消化率和回肠绒毛高度 (VH) (p ≤ .05)。在日粮中添加 0.5 g/kg BA 会增加垫料水分、血清钙含量、空肠绒毛高度和回肠绒毛宽度 (VW) (p ≤ .05)。然而，补充 Phy 和 BA 的组合降低了回肠 Ca 的表观消化率（2X），并增加了空肠的 VH/CD 比（1X）。总之，Phy 在改善性能特征、骨矿化、表观回肠营养物质消化率和肠道形态方面是有效的。BA 对性能特征、骨矿化和回肠营养物质消化率没有影响，但在推荐水平 (0.5 g/kg) 下会增加回肠和空肠的 VH 和 VW。亮点 在缺乏营养的日粮中添加植酸酶可显着改善雄性肉鸡的体重和增重。补充植酸酶可增加血清磷含量并降低血清钙和垫料水分含量。补充植酸酶增加了胫骨长度和灰分含量、磷的表观回肠消化率和回肠绒毛高度。在营养缺乏的日粮中添加苯甲酸会增加垫料水分、血清钙含量、空肠绒毛高度和回肠绒毛宽度。植酸酶和苯甲酸补充剂的组合分别降低了钙的表观回肠消化率、回肠的隐窝深度和增加的空肠和回肠的绒毛宽度和绒毛高度/隐窝深度比。补充植酸酶可增加血清磷含量并降低血清钙和垫料水分含量。补充植酸酶增加了胫骨长度和灰分含量、磷的表观回肠消化率和回肠绒毛高度。在营养缺乏的日粮中添加苯甲酸会增加垫料水分、血清钙含量、空肠绒毛高度和回肠绒毛宽度。植酸酶和苯甲酸补充剂的组合分别降低了钙的表观回肠消化率、回肠的隐窝深度和增加的空肠和回肠的绒毛宽度和绒毛高度/隐窝深度比。补充植酸酶可增加血清磷含量并降低血清钙和垫料水分含量。补充植酸酶增加了胫骨长度和灰分含量、磷的表观回肠消化率和回肠绒毛高度。在营养缺乏的日粮中添加苯甲酸会增加垫料水分、血清钙含量、空肠绒毛高度和回肠绒毛宽度。植酸酶和苯甲酸补充剂的组合分别降低了钙的表观回肠消化率、回肠的隐窝深度和增加的空肠和回肠的绒毛宽度和绒毛高度/隐窝深度比。磷的表观回肠消化率和回肠绒毛高度。在营养缺乏的日粮中添加苯甲酸会增加垫料水分、血清钙含量、空肠绒毛高度和回肠绒毛宽度。植酸酶和苯甲酸补充剂的组合分别降低了钙的表观回肠消化率、回肠的隐窝深度和增加的空肠和回肠的绒毛宽度和绒毛高度/隐窝深度比。磷的表观回肠消化率和回肠绒毛高度。在营养缺乏的日粮中添加苯甲酸会增加垫料水分、血清钙含量、空肠绒毛高度和回肠绒毛宽度。植酸酶和苯甲酸补充剂的组合分别降低了钙的表观回肠消化率、回肠的隐窝深度和增加的空肠和回肠的绒毛宽度和绒毛高度/隐窝深度比。