ABSTRACT

Introduction: Antibiotic resistance is widely found even among bacterial populations not having been exposed to selective pressure by antibiotics, such as tetracycline. In this study we analyzed the tetracycline-resistant subgingival microbiota of healthy subjects and of patients with periodontitis, comparing the prevalence of tet genes and their multidrug resistance profiles.

Methods: Samples from 259 volunteers were analyzed, obtaining 813 tetracycline-resistant isolates. The prevalence of 12 antibiotic resistance genes was assessed, and multidrug profiles were built. Each isolate was identified by 16S rRNA sequencing. Differences in qualitative data and quantitative data were evaluated using the chi-square test and the Mann-Whitney-U test, respectively.

Results: tet(M) was the most frequently detected tet gene (52.03%). We observed significant differences between the prevalence of tet(M), tet(W), tet(O), tet(32) and tet(L) in both populations studied. Multidrug resistance was largely observed, with resistance to kanamycin being the most detected (83.64%). There were significant differences between the populations in the prevalence of kanamycin, chloramphenicol, and cefotaxime resistance. Resistant isolates showed significantly different prevalence between the two studied groups.

Conclusion: The high prevalence of multidrug resistance and tetracycline resistance genes found in the subgingival microbiota, highlights the importance of performing wider and more in-depth analysis of antibiotic resistance in the oral microbiota.

摘要

简介：即使在未受到抗生素（例如四环素）选择压力的细菌种群中，也广泛发现了抗生素耐药性。在这项研究中，我们分析了健康受试者和牙周炎患者的四环素耐药性龈下菌群，比较了tet基因的患病率及其多药耐药性。

方法：对259名志愿者的样本进行分析，获得813株抗四环素的菌株。评估了12种抗生素抗性基因的患病率，并建立了多药谱。通过16S rRNA测序鉴定每个分离物。使用卡方检验和Mann-Whitney-U检验分别评估定性数据和定量数据的差异。

结果：tet（M）是检测到频率最高的tet基因（52.03％）。我们在两个研究人群中观察到tet（M），tet（W），tet（O），tet（32）和tet（L）的患病率之间存在显着差异。观察到多药耐药性，对卡那霉素的耐药性最高（83.64％）。人群之间卡那霉素，氯霉素和头孢噻肟耐药的发生率存在显着差异。耐药菌株在两个研究组之间显示出明显不同的患病率。

结论：在龈下菌群中发现了多药耐药和四环素耐药基因，这凸显了在口腔菌群中进行更广泛，更深入的抗生素耐药性分析的重要性。