Some plant-specific resistance genes could affect rhizosphere microorganisms by regulating the release of root exudates. In a previous study, the SST (seedling salt tolerant) gene in rice (Oryza sativa) was identified, and loss of SST function resulted in better plant adaptation to salt stress. However, whether the rice SST variation could alleviate salt stress via regulating soil metabolites and microbiota in the rhizosphere is still unknown. Here, we used transgenic plants with SST edited in the Huanghuazhan (HHZ) and Zhonghua 11 (ZH11) cultivars by the CRISPR/Cas9 system and found that loss of SST function increased the accumulation of potassium and reduced the accumulation of sodium ions in rice plants. Using 16S rRNA gene amplicon high-throughput sequencing, we found that the mutant material shifted the rhizobacterial assembly under salt-free stress. Importantly, under salt stress, the sst, HHZcas, and ZH11cas plants significantly changed the assembly of the rhizobacteria. Furthermore, the rice SST gene also affected the soil metabolites, which were closely related to the dynamics of rhizosphere microbial communities, and we further determined the relationship between the rhizosphere microbiota and soil metabolites. Overall, our results show the effects of the rice SST gene on the response to salt stress associated with the soil microbiota and metabolites in the rhizosphere. This study reveals a helpful linkage among the rice SST gene, soil metabolites, and rhizobacterial community assembly and also provides a theoretical basis for improving crop adaptation through soil microbial management practices.

中文翻译：

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水稻SST变异塑造了根际细菌群落，通过调节土壤代谢物赋予了对盐胁迫的耐受性

一些植物特异性抗性基因可能通过调节根系分泌物的释放而影响根际微生物。在先前的研究中，鉴定了水稻（水稻）的SST（耐盐幼苗）基因，并且SST功能的丧失导致植物对盐胁迫的适应性更好。但是，水稻SST的变化是否能够通过调节根际土壤代谢物和微生物群落来缓解盐胁迫尚不清楚。在这里，我们使用通过CRISPR / Cas9系统在黄花站（HHZ）和中华11（ZH11）品种中编辑过SST的转基因植物，发现SST的缺失功能增加了水稻植株中钾的积累，减少了钠离子的积累。使用16S rRNA基因扩增子高通量测序，我们发现突变材料在无盐胁迫下转移了根瘤菌的组装。重要的是，在盐胁迫下，sst，HHZ cas和ZH11 cas植物显着改变了根瘤菌的装配。此外，水稻SST基因也影响了土壤代谢产物，这与根际微生物群落的动力学密切相关，我们进一步确定了根际微生物群与土壤代谢产物之间的关系。总体而言，我们的结果显示了稻米SST的影响基因对根际土壤微生物和代谢产物相关的盐胁迫的响应。这项研究揭示了水稻SST基因，土壤代谢产物和根瘤菌群落组装之间的有益联系，也为通过土壤微生物管理实践改善作物适应性提供了理论基础。