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Biocomposite scaffolds for 3D cell culture: Propolis enriched polyvinyl alcohol nanofibers favoring cell adhesion
Journal of Applied Polymer Science ( IF 2.7 ) Pub Date : 2020-11-24 , DOI: 10.1002/app.50287 Rumeysa Bilginer 1 , Dilce Ozkendir‐Inanc 2 , Umit Hakan Yildiz 3 , Ahu Arslan‐Yildiz 1
Journal of Applied Polymer Science ( IF 2.7 ) Pub Date : 2020-11-24 , DOI: 10.1002/app.50287 Rumeysa Bilginer 1 , Dilce Ozkendir‐Inanc 2 , Umit Hakan Yildiz 3 , Ahu Arslan‐Yildiz 1
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The objective of this work is generation of propolis/polyvinyl alcohol (PVA) scaffold by electrospinning for 3D cell culture. Here, PVA used as co‐spinning agent since propolis alone cannot be easily processed by electrospinning methodology. Propolis takes charge in maximizing biological aspect of scaffold to facilitate cell attachment and proliferation. Morphological analysis showed size of the electrospun nanofibers varied between 172–523 nm and 345–687 nm in diameter, for non‐crosslinked and crosslinked scaffolds, respectively. Incorporation of propolis resulted in desired surface properties of hybrid matrix, where hybrid scaffolds highly favored protein adsorption. To examine cell compatibility, NIH‐3T3 and HeLa cells were seeded on propolis/PVA hybrid scaffold. Results confirmed that integration of propolis supported cell adhesion and cell proliferation. Also, results indicated electrospun propolis/PVA hybrid scaffold provide suitable microenvironment for cell culturing. Therefore, developed hybrid scaffold could be considered as potential candidate for 3D cell culture and tissue engineering.
中文翻译：
用于3D细胞培养的生物复合支架：富含蜂胶的聚乙烯醇纳米纤维，有利于细胞粘附
这项工作的目的是通过静电纺丝生产3D细胞培养蜂胶/聚乙烯醇（PVA）支架。在这里，PVA用作助纺剂，因为单独的蜂胶无法通过电纺方法轻松加工。蜂胶负责使支架的生物学最大化，以促进细胞附着和增殖。形态分析表明，电纺纳米纤维的大小分别在未交联和交联的支架上，直径在172–523 nm和345–687 nm之间。蜂胶的掺入产生了混合基质所需的表面特性，其中混合支架高度有利于蛋白质吸附。为了检查细胞相容性，将NIH-3T3和HeLa细胞接种在蜂胶/ PVA混合支架上。结果证实蜂胶的整合支持细胞粘附和细胞增殖。同样，结果表明，静电纺丝蜂胶/ PVA混合支架为细胞培养提供了合适的微环境。因此，开发的混合支架可以被认为是3D细胞培养和组织工程的潜在候选者。
更新日期：2021-02-01
中文翻译：
用于3D细胞培养的生物复合支架：富含蜂胶的聚乙烯醇纳米纤维，有利于细胞粘附
这项工作的目的是通过静电纺丝生产3D细胞培养蜂胶/聚乙烯醇（PVA）支架。在这里，PVA用作助纺剂，因为单独的蜂胶无法通过电纺方法轻松加工。蜂胶负责使支架的生物学最大化，以促进细胞附着和增殖。形态分析表明，电纺纳米纤维的大小分别在未交联和交联的支架上，直径在172–523 nm和345–687 nm之间。蜂胶的掺入产生了混合基质所需的表面特性，其中混合支架高度有利于蛋白质吸附。为了检查细胞相容性，将NIH-3T3和HeLa细胞接种在蜂胶/ PVA混合支架上。结果证实蜂胶的整合支持细胞粘附和细胞增殖。同样，结果表明，静电纺丝蜂胶/ PVA混合支架为细胞培养提供了合适的微环境。因此，开发的混合支架可以被认为是3D细胞培养和组织工程的潜在候选者。
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