Drought is a limiting factor for wheat production. Nitric oxide (NO) and hydrogen peroxide (H₂O₂) are key signalling molecules produced in response to drought stress. Drought priming is a promising strategy for enhancing drought tolerance. Here, the roles of NO and H₂O₂ in drought priming were investigated. The results showed that the scavengers of NO or H₂O₂ reduced the effects of priming‐induced tolerance to drought stress during seedling and grain filling stages. Drought priming induced the production of H₂O₂ and NO, suggesting that H₂O₂ and NO were involved in priming‐induced drought tolerance and that the synthesis of H₂O₂ could be related to respiratory burst oxidase homologues (RBOH). Further, nitrate reductase (NR) activity was not the unique source for NO production. The elevated production of NO in the primed plants was blocked by scavengers of both H₂O₂ and NO, whereas the H₂O₂ concentration was not affected by a scavenger of NO. Osmolyte accumulation induced by drought priming was partly inhibited by the removal of NO, but was higher than in non‐primed plants. NO worked downstream of H₂O₂‐mediated the accumulation of proline and glycine‐betaine in primed plants under drought stress during grain filling. The results increase our understanding of the roles of H₂O₂ and NO in priming‐induced drought tolerance in wheat.

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过氧化氢和一氧化氮之间的串扰介导了小麦引发的耐旱性

干旱是小麦生产的限制因素。一氧化氮（NO）和过氧化氢（H ₂ O ₂）是响应干旱胁迫而产生的关键信号分子。引发干旱是增强抗旱性的有前途的策略。在此，研究了NO和H ₂ O ₂在干旱引发中的作用。结果表明，NO或H ₂ O ₂的清除剂降低了在苗期和灌浆期引发引发的对干旱胁迫的耐受性。干旱引发诱导了H ₂ O ₂和NO的产生，表明H ₂ O ₂和NO参与引发的干旱耐受性，H ₂ O ₂的合成可能与呼吸爆发氧化酶同源物（RBOH）有关。此外，硝酸盐还原酶（NR）活性不是NO产生的唯一来源。引发植物中NO的高产量被H ₂ O ₂和NO的清除剂阻止，而H ₂ O _2的浓度不受NO清除剂的影响。干旱引发的渗透液积累被NO的去除部分抑制，但高于未引发的植物。H ₂ O ₂下游没有工作介导的干旱胁迫下，发芽植物中脯氨酸和甘氨酸-甜菜碱的积累。结果加深了我们对H ₂ O ₂和NO在启动引发的小麦耐旱性中的作用的了解。