This study demonstrated a facile, green and bioinspired approach to synthesize protein-posnjakite nanobiohybrid with rod-assembled hollow shuttle-like structure. Through the one-pot mild coprecipitation process, the inorganic mineral posnjakite (Cu4(SO4) (OH)6·H2O) served as a nanocarrier to efficient co-immobilization of recognition protein (streptavidin) and enzyme (horseradish peroxidase) for signal amplification, which avoids tedious linking or purification procedures and significantly simplifies the synthetic process. This nanobiohybrid was then utilized as the signal tag for immunoassays and presented excellent performance for the detection of insecticidal crystalline (Cry) protein Cry1Ab, in the linear range of 0.1-40 ng mL-1, with the limit of detection of 63 pg mL-1. This proposed strategy is expected to the integration of a variety of biomolecules with posnjakite to design diverse multifunctional nanobiohybrids for multiple applications extending from biosensors, catalysis and biomedicine to environmental science and energy.

中文翻译：

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用于生物传感应用的蛋白质-posnjakite 有机-无机纳米生物杂化物的仿生合成

这项研究展示了一种简便、绿色和仿生的方法来合成具有棒状组装空心梭状结构的蛋白质-posnjakite 纳米生物杂化物。通过一锅温和的共沉淀过程，无机矿物posnjakite（Cu4（SO4）（OH）6·H2O）作为纳米载体，有效共固定识别蛋白（链霉亲和素）和酶（辣根过氧化物酶）进行信号放大，这避免了繁琐的连接或纯化程序，并显着简化了合成过程。然后将该纳米生物杂化物用作免疫测定的信号标签，并在检测杀虫结晶 (Cry) 蛋白 Cry1Ab 方面表现出色，线性范围为 0.1-40 ng mL-1，检测限为 63 pg mL- 1.