Abstract

This work was aimed at compiling the world’s first linkage map of redcurrant with the use of microsatellite and SNP DNA markers. As a result of mapping, eight linkage groups with an LOD level of 10 were constructed, which included 299 DNA markers, including 12 microsatellite and 287 SNP markers; the total length of all linkage groups was 741 cM; the average was 93 cM. In seven of the eight linkage groups obtained, at least one microsatellite DNA marker was mapped. Most of the microsatellite loci localized on the redcurrant map correspond to their position on the blackcurrant linkage groups (loci e3-B02, g1-A01, and g1-L12 are located on the same linkage group no. 5; loci g2-J08, g2-B20, e1-O21, and e1-O01 on the resulting map are each in a separate linkage group 2, 3, 4, and 6, respectively), with the exception of the loci mapped at blackcurrant on linkage group 1, which in our work were unmapped. The g2-J11 locus in our studies amplifies fragments in two ranges, which suggests its duplication in the genome of the studied samples. This locus was mapped in two linkage groups of redcurrant. Two new, not previously mapped and unpublished microsatellite loci were also localized on linkage map of redcurrant. In the future, it is planned to localize economically useful traits in the redcurrant genome.

中文翻译：

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利用SSR和SNP DNA标记开发红浆果（Ribes rubrum L.）的遗传连锁图谱

摘要

这项工作旨在利用微卫星和SNP DNA标记物编制世界上第一个红醋栗连锁图。作为作图的结果，构建了8个LOD水平的连锁基团，包括299个DNA标记，其中包括12个微卫星和287个SNP标记。所有连锁组的总长度为741 cM；平均为93 cM。在获得的八个连接基团中的七个中，绘制了至少一个微卫星DNA标记。定位在红浆果图上的大多数微卫星基因座对应于它们在黑醋栗连锁组上的位置（位置e3-B02，g1-A01和g1-L12位于同一连锁组5；基因座g2-J08，g2生成图上的-B20，e1-O21和e1-O01分别位于单独的链接组2、3、4和6中，除了映射到链接组1上黑加仑子上的基因座以外，我们的工作中未映射这些基因座。我们研究中的g2-J11基因座在两个范围内扩增了片段，这表明它在研究样品的基因组中重复。该基因座被映射在红浆果的两个连锁群中。两个新的，以前未映射且未发布的微卫星基因座也位于红浆果连锁图上。将来，计划在红浆果基因组中定位经济上有用的性状。以前未映射且未发布的微卫星基因座也位于红浆果连锁图上。将来，计划在红浆果基因组中定位经济上有用的性状。以前未映射且未发布的微卫星基因座也位于红浆果连锁图上。将来，计划在红浆果基因组中定位经济上有用的性状。