A total of 60 healthy (the control group) and 30 anestrous Holstein cows with inactive ovaries (the case group) from three dairy farms around Tehran were randomly selected and diagnosed to determine the concentrations of glucose, insulin, and cortisol, and the rate of the insulin resistance disorder in them. Primarily, serum samples were obtained from all animals, and the concentrations of glucose, insulin, and cortisol were measured in a laboratory. Thereupon, the glucose tolerance test (GTT) was implemented. Before the implementation of the GTT, the median of serum glucose levels (62 mg/dl), insulin (7.16 μIU/ml), and cortisol (2.5 μg/dl) was estimated in healthy cows. The median of serum glucose levels (68.5 mg/dl, p = 0.00009) was higher, and serum insulin (6.1 μIU/ml, p = 0.025) and cortisol (1.1 μg/dl, p = 0.00029) levels were lesser in the anestrous cows when estimated simultaneously. After the implementation of GTT, the median of serum glucose concentrations in one (120 mg/dl), two (73.5 mg/dl), and three (63.5 mg/dl) hours after the glucose injection was estimated in healthy cows. The findings also showed that the median of serum glucose levels was lesser in one (100 mg/dl, p = 0.015), similar in two (77.5 mg/dl, p = 0.9), and higher in three (70 mg/dl, p = 0.012) hours after the glucose injection in anestrous cows. In conclusion, higher serum glucose, lesser insulin and cortisol concentrations, and the different correlation between serum glucose and insulin levels before the GTT were detected in anestrous cows. The pattern of glucose changes also differed in anestrous cows after the GTT.

从德黑兰附近的三个奶牛场中随机选择了60头健康的（对照组）健康的卵巢无卵巢荷斯坦奶牛（病例组），并对其进行了诊断，以确定葡萄糖，胰岛素和皮质醇的浓度以及他们的胰岛素抵抗障碍。首先，从所有动物获得血清样品，并在实验室中测量葡萄糖，胰岛素和皮质醇的浓度。随即，进行了葡萄糖耐量试验（GTT）。在实施GTT之前，估计健康母牛的血清葡萄糖水平（62 mg / dl），胰岛素（7.16μIU/ ml）和皮质醇（2.5μg/ dl）的中位数。血清葡萄糖水平的中位数（68.5 mg / dl，p  = 0.00009）更高，血清胰岛素（6.1μIU/ ml，p 当同时估算时，有情奶牛的 皮质醇（0.025）和皮质醇（1.1μg/ dl，p = 0.00029）含量较低。实施GTT后，估计健康母牛的葡萄糖注射后一小时（120 mg / dl），两小时（73.5 mg / dl）和三小时（63.5 mg / dl）的血清葡萄糖浓度中位数。研究结果还显示，血清葡萄糖水平的中位数在一种（100 mg / dl，p  = 0.015）中较低，在两种（77.5 mg / dl，p  = 0.9）中相似，而在三种（70 mg / dl，p =在发情的母牛注射葡萄糖后= 0.012）小时。总之，在发情的奶牛中检测到较高的血清葡萄糖，较低的胰岛素和皮质醇浓度，以及在GTT前血清葡萄糖和胰岛素水平之间的相关性不同。GTT后，在发情期母牛中，葡萄糖变化的模式也不同。