Key message

Piriformospora indica symbiosis promoted the growth and photosynthesis, and simultaneously enhanced the resistance against insect herbivory by regulating sporamin-dependent defense in sweet potato.

Abstract

Piriformospora indica (P. indica), a versatile endophytic fungus, promotes the growth and confers resistance against multiple stresses by root colonization in plant hosts. In this study, the effects of P. indica colonization on the growth, physiological change, and herbivore resistance of leaf-vegetable sweet potato cultivar were investigated. P. indica symbiosis significantly improved the biomass in both above- and under-ground parts of sweet potato plants. In comparison with the non-colonized plants, the content of photosynthetic pigments and the efficiency of photosynthesis were increased in P. indica-colonized sweet potato plants. Further investigation showed that the activity of catalase was enhanced in both leaves and roots of sweet potato plants after colonization, but ascorbate peroxidase, peroxidase, and superoxide dismutase were not enhanced. Furthermore, the interaction between P. indica and sweet potato plants also showed the biological function in jasmonic acid (JA)-mediated defense. The plants colonized by P. indica had greatly increased JA accumulation and defense gene expressions, including IbNAC1, IbbHLH3, IbpreproHypSys, and sporamin, leading to elevated trypsin inhibitory activity, which was consistent with a reduced Spodoptera litura performance when larvae fed on the leaves of P. indica-colonized sweet potato plants. The root symbiosis of P. indica is helpful for the plant promoting growth and development and has a strong function as resistance inducers against herbivore attack in sweet potato cultivation by regulating sporamin-dependent defense.

中文翻译：

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Piriformospora indica 定植增加甘薯 ( Ipomoea batatas L.) 的生长、发育和草食性抗性

关键信息

Piriformospora indica共生促进了甘薯的生长和光合作用，同时通过调节甘薯的 sporamin 依赖性防御增强了对食草昆虫的抵抗力。

抽象的

Piriformospora indica ( P. indica ) 是一种多用途的内生真菌，通过在植物宿主中的根定植促进生长并赋予对多种胁迫的抵抗力。在本研究中，研究了P. indica定殖对叶菜甘薯品种生长、生理变化和食草动物抗性的影响。P. indica共生显着提高了甘薯植物地上和地下部分的生物量。与未定殖的植物相比，籼稻的光合色素含量和光合作用效率均有所提高。-殖民的甘薯植物。进一步研究表明，定殖后甘薯植株叶片和根部的过氧化氢酶活性增强，但抗坏血酸过氧化物酶、过氧化物酶和超氧化物歧化酶没有增强。此外， P. indica和甘薯植物之间的相互作用也显示出茉莉酸 (JA) 介导的防御的生物学功能。被P. indica定殖的植物的JA 积累和防御基因表达显着增加，包括IbNAC1、IbbHLH3、IbpreproHypSys和sporamin，导致胰蛋白酶抑制活性升高，这与幼虫以 Spodoptera litura 的叶子为食时降低的斜纹夜蛾性能一致。P. indica - 定殖的甘薯植物。P. indica的根共生有助于植物的生长发育，并通过调节sporamin依赖性防御，在甘薯栽培中作为抗食草动物攻击的抗性诱导剂具有很强的作用。