Temporal lobe epilepsy (TLE) is common intractable epilepsy that affects the patient’s lives. The circular RNA circ_ANKMY2 (circ_ANKMY2) has been reported to be abnormally expressed in TLE. Nevertheless, the role and mechanism of circ_ANKMY2 in TLE are unclear. A human neuroblastoma cell line (SK-N-AS) was used for a series of studies. Expression levels of circ_ANKMY2, miR-106b-5p, and Forkhead Box Protein 1 (FOXP1) mRNA in TLE tissues were assessed through quantitative real-time polymerase chain reaction (qRT-PCR). Cell colony formation, proliferation, and apoptosis were determined by cell colony formation, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), or flow cytometry assays. The levels of FOXP1 protein, Ki67, B cell lymphoma (Bcl-2), Bcl-2 Associated X (Bax), and Cleaved caspase-3 were evaluated by western blot analysis. The relationship between circ_ANKMY2 or FOXP1 and miR-106b-5p was verified with dual-luciferase reporter assay. We observed that circ_ANKMY2 and FOXP1 expression were reduced while miR-106b-5p expression was increased in TLE tissues. Overexpression of circ_ANKMY2 decreased spontaneous recurrent seizures (SRSs) in rat TLE model and blocked cell colony formation, proliferation, and induced cell apoptosis in SK-N-AS cells. Importantly, circ_ANKMY2 was verified as a sponge for miR-106b-5p. In addition, miR-106b-5p mimics abolished circ_ANKMY2 elevation-mediated effects on colony formation, proliferation, and apoptosis of SK-N-AS cells. Also, FOXP1 served as a target for miR-106b-5p. And FOXP1 silencing overturned the effects of miR-106b-5p inhibitors on the colony formation, proliferation, and apoptosis of SK-N-AS cells. In sum, circ_ANKMY2 modulated TLE advancement via regulation of FOXP1 expression through sponging miR-106b-5p, and circ_ANKMY2 might be an underlying target for the improvement of TLE.

颞叶癫痫病（TLE）是常见的难治性癫痫病，会影响患者的生活。据报道，环状RNA circ_ANKMY2（circ_ANKMY2）在TLE中异常表达。然而，尚不清楚circ_ANKMY2在TLE中的作用和机制。人类神经母细胞瘤细胞系（SK-N-AS）用于一系列研究。通过定量实时聚合酶链反应（qRT-PCR）评估TLE组织中circ_ANKMY2，miR-106b-5p和Forkhead Box Protein 1（FOXP1）mRNA的表达水平。细胞集落形成，增殖和凋亡通过细胞集落形成，3-（4,5-二甲基噻唑-2-基）-2,5-二苯基四唑溴化物（MTT）或流式细胞术测定来确定。通过蛋白质印迹分析评估了FOXP1蛋白，Ki67，B细胞淋巴瘤（Bcl-2），Bcl-2相关X（Bax）和Cleaved caspase-3的水平。用双荧光素酶报告基因分析证实了circ_ANKMY2或FOXP1与miR-106b-5p之间的关系。我们观察到，TLE组织中circ_ANKMY2和FOXP1表达降低，而miR-106b-5p表达升高。circ_ANKMY2的过表达减少了大鼠TLE模型中的自发性复发性癫痫发作（SRS），并阻止了SK-N-AS细胞中的细胞集落形成，增殖和诱导的细胞凋亡。重要的是，验证了circ_ANKMY2作为miR-106b-5p的海绵。另外，miR-106b-5p消除了circ_ANKMY2升高介导的SK-N-AS细胞集落形成，增殖和凋亡的作用。此外，FOXP1也是miR-106b-5p的目标。FOXP1沉默消除了miR-106b-5p抑制剂对SK-N-AS细胞集落形成，增殖和凋亡的影响。总共，