In this study, human skin fibroblasts human dermis fibroblasts were used as the object, the possible effects and mechanisms of rosemary extract on repeated exposure to Ultraviolet A (UVA) induced senescence of human skin fibroblasts were investigated. During the research, we mainly carried out through two parts. The first part mainly explores the effect of repeated UVA radiation on human skin fibroblasts; the second part mainly discusses the effect of rosemary extract on the induction of human skin formation by repeated UVA radiation. The results showed that repeated UVA irradiation of human skin fibroblasts can induce aging phenomena, such as slow proliferation and an increase in β-galactosidase staining positive rate; repeated exposure of rosemary extract to low-dose UVA can slow down human skin fibroblasts to age. Its mechanism may be related to reducing the total intracellular ROS, the mRNA expression of MMP-1 and MMP-3, and the expression of p53 protein. In short, this study provides a new approach and theoretical basis for the development and application of natural sunscreens.

本研究以人皮肤成纤维细胞为研究对象，研究了迷迭香提取物反复暴露于紫外线A（UVA）诱导人皮肤成纤维细胞衰老的可能作用及其机制。在研究过程中，我们主要分两个部分进行。第一部分主要探讨重复UVA辐射对人皮肤成纤维细胞的影响。第二部分主要讨论了迷迭香提取物通过重复的UVA辐射对人体皮肤形成的诱导作用。结果表明，人皮肤成纤维细胞的反复UVA照射可引起衰老现象，例如增殖缓慢和β-半乳糖苷酶染色阳性率的增加。迷迭香提取物反复暴露于低剂量UVA会减慢人类皮肤成纤维细胞的衰老速度。其机制可能与降低细胞内总ROS，降低MMP-1和MMP-3的mRNA表达以及p53蛋白的表达有关。简而言之，本研究为天然防晒剂的开发和应用提供了新的方法和理论基础。