A real-time dPCR system was developed to improve the sensitivity, specificity and quantification accuracy of end point dPCR. We compared three technologies - real-time qPCR, end point dPCR and real-time dPCR - in the context of SARS-CoV-2. Some improvement in limit of detection was obtained with end point dPCR compared with real-time qPCR, and the limit of detection was further improved with the newly developed real-time dPCR technology through removal of false-positive signals. Real-time dPCR showed increased linear dynamic range compared with end point dPCR based on quantitation from amplification curves. Real-time dPCR can improve the performance of TaqMan assays beyond real-time qPCR and end point dPCR with better sensitivity and specificity, absolute quantification and a wider linear range of detection.

中文翻译：

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使用实时dPCR检测SARS-CoV-2可提高灵敏度。

开发了实时dPCR系统以提高终点dPCR的灵敏度，特异性和定量准确性。我们在SARS-CoV-2的背景下比较了三种技术-实时定量PCR，终点dPCR和实时dPCR。与实时qPCR相比，终点dPCR的检测限得到了一些改善，新开发的实时dPCR技术通过去除假阳性信号进一步提高了检测限。与基于扩增曲线定量的终点dPCR相比，实时dPCR显示出更高的线性动态范围。实时dPCR可以提高TaqMan分析的性能，使其优于实时qPCR和终点dPCR，具有更好的灵敏度和特异性，绝对定量和更宽的线性检测范围。