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Biomolecules from snail mucus (Helix aspersa) conjugated gold nanoparticles, exhibiting potential wound healing and anti-inflammatory activity
Soft Matter ( IF 2.9 ) Pub Date : 2020-11-05 , DOI: 10.1039/d0sm01638a Jennifer Gubitosa 1 , Vito Rizzi , Paola Fini , Anna Laurenzana , Gabriella Fibbi , Clara Veiga-Villauriz , Fiorenza Fanelli , Francesco Fracassi , Alberto Onzo , Giuliana Bianco , Carmine Gaeta , Antonio Guerrieri , Pinalysa Cosma
Soft Matter ( IF 2.9 ) Pub Date : 2020-11-05 , DOI: 10.1039/d0sm01638a Jennifer Gubitosa 1 , Vito Rizzi , Paola Fini , Anna Laurenzana , Gabriella Fibbi , Clara Veiga-Villauriz , Fiorenza Fanelli , Francesco Fracassi , Alberto Onzo , Giuliana Bianco , Carmine Gaeta , Antonio Guerrieri , Pinalysa Cosma
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In this work, for the first time, snail slime from garden snails “Helix Aspersa Müller”, has been used to induce the formation of eco-friendly gold nanoparticles (AuNPs-SS) suitable for biomedical applications. An AuNPs-SS comprehensive investigation was performed and AuNPs with an average particle size of 14 ± 6 nm were observed, stabilized by a slime snail-based organic layer. Indeed, as recognized in high-resolution MALDI-MS analyses, and corroborated by FESEM, UV-Vis, ATR-FTIR, and XPS results, it was possible to assess the main presence of peptides and amino acids as the main components of the slime, that, combined with the AuNPs confers on them interesting properties. More specifically, we tested, in vitro, the AuNPs-SS safety in human keratinocytes and their potential effect on wound healing as well as their anti-inflammatory properties in murine macrophages. Moreover, the AuNPs-SS treatment resulted in a significant increase of the urokinase-type plasminogen activator receptor (uPAR), essential for keratinocyte adhesion, spreading, and migration, together with the reduction of LPS-induced IL1-β and IL-6 cytokine levels, and completely abrogated the synthesis of inducible nitric oxide synthase (iNOS).
中文翻译：
蜗牛黏液（Helix aspersa）结合的金纳米颗粒生物分子，具有潜在的伤口愈合和抗炎活性
在这项工作中，花园蜗牛“ Helix AspersaMüller ”的蜗牛泥首次被用于诱导形成适用于生物医学应用的环保金纳米颗粒（AuNPs-SS）。进行了AuNPs-SS的综合研究，观察到平均粒径为14±6 nm的AuNPs，并通过粘液蜗牛基有机层使其稳定。实际上，正如高分辨率MALDI-MS分析所公认的，并得到FESEM，UV-Vis，ATR-FTIR和XPS结果的证实，有可能评估作为粘液主要成分的肽和氨基酸的主要存在，再加上AuNP赋予它们有趣的特性。更具体地说，我们在体外进行了测试，AuNPs-SS在人角质形成细胞中的安全性及其对伤口愈合的潜在影响以及在小鼠巨噬细胞中的抗炎特性。此外，AuNPs-SS治疗导致尿激酶型纤溶酶原激活剂受体（uPAR）显着增加，这对于角质形成细胞的粘附，扩散和迁移至关重要，同时还降低了LPS诱导的IL1-β和IL-6细胞因子。水平，并完全废除了诱导型一氧化氮合酶（iNOS）的合成。
更新日期：2020-11-23
中文翻译：
蜗牛黏液（Helix aspersa）结合的金纳米颗粒生物分子，具有潜在的伤口愈合和抗炎活性
在这项工作中，花园蜗牛“ Helix AspersaMüller ”的蜗牛泥首次被用于诱导形成适用于生物医学应用的环保金纳米颗粒（AuNPs-SS）。进行了AuNPs-SS的综合研究，观察到平均粒径为14±6 nm的AuNPs，并通过粘液蜗牛基有机层使其稳定。实际上，正如高分辨率MALDI-MS分析所公认的，并得到FESEM，UV-Vis，ATR-FTIR和XPS结果的证实，有可能评估作为粘液主要成分的肽和氨基酸的主要存在，再加上AuNP赋予它们有趣的特性。更具体地说，我们在体外进行了测试，AuNPs-SS在人角质形成细胞中的安全性及其对伤口愈合的潜在影响以及在小鼠巨噬细胞中的抗炎特性。此外，AuNPs-SS治疗导致尿激酶型纤溶酶原激活剂受体（uPAR）显着增加，这对于角质形成细胞的粘附，扩散和迁移至关重要，同时还降低了LPS诱导的IL1-β和IL-6细胞因子。水平，并完全废除了诱导型一氧化氮合酶（iNOS）的合成。
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