The present study was designed to investigate the in vivo wound healing activity of herbal ointment prepared from Calendula officinalis Linn. on excision wounded rats. The excision wound model was employed for wound healing activity in albino rats. Healthy albino rats (150–200 g) of either sex were taken for excision wound model. Animals were divided into five groups of six animals in each. Group I served as normal control, Group II served as excision wounded control without treatment and Group III, IV served as excision wounded rats were treated with herbal ointment of two different doses (10% and 20%) applied topically for 14 days and group V served as excision wounded animals treated with reference ointment soframycin. Healing potential was evaluated by the rate of wound contraction, immunological markers like IL-6(Interleukin 6), TNF-alpha (Tumor necrosis factor -α), PDGF (Platelet Derived Growth Factor) and EGF (Epidermal Growth Factor), lipid peroxide (LPO), superoxide dismutase (SOD), and biochemical parameters like hydroxyproline, hesosamine, and tissue protein. The topical application of herbal ointment treated groups showed increase in the levels of growth factors such as PDGF and EGF hydroxy proline, hesosamine, tissue protein, SOD and wound contraction and the ointment normalized the levels of lipid peroxide, IL-6, TNF-alpha compared than that of excision wounded animals. From the above results, it was concluded that the topical application of herbal ointment exhibited significant wound healing activity in excision wounded rats as evidenced by increased wound contraction and collagen synthesis.

中文翻译：

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含有金盏菊的药膏的伤口愈合潜力。受伤动物免疫学指标和生化指标的变化

本研究旨在研究从金盏菊制备的草药软膏的体内伤口愈合活性。在切除受伤的大鼠上。切除伤口模型用于白化病大鼠的伤口愈合活性。取健康的两性白化病大鼠（150–200 g）作为切除伤口模型。将动物分为五组，每组六只。第一组作为正常对照组，第二组作为未经治疗的切除伤口的对照组，第三，第四组作为切除伤口的大鼠，分别用两种不同剂量（10％和20％）的药膏软膏治疗，持续14天，第五组用作用参比霉素霉素软膏治疗的切除伤口的动物。通过伤口收缩率，IL-6（Interleukin 6）等免疫学标记，TNF-α（肿瘤坏死因子-α），PDGF（血小板衍生生长因子）和EGF（表皮生长因子），脂质过氧化物（LPO），超氧化物歧化酶（SOD）以及生化参数，例如羟脯氨酸，杂粮胺和组织蛋白。药膏治疗组的局部应用表明，PDGF和EGF羟基脯氨酸，he素，组织蛋白，SOD和伤口收缩等生长因子的水平增加，并且该药膏使脂质过氧化物，IL-6，TNF-α的水平正常化比切除受伤的动物要大。从以上结果可以得出结论，局部伤口愈合和胶原合成增加证明，局部应用草药软膏在切除伤口的大鼠中具有明显的伤口愈合活性。PDGF（血小板衍生生长因子）和EGF（表皮生长因子），脂质过氧化物（LPO），超氧化物歧化酶（SOD）以及生化参数，例如羟脯氨酸，杂糖胺和组织蛋白。药膏治疗组的局部应用表明，PDGF和EGF羟基脯氨酸，he素，组织蛋白，SOD和伤口收缩等生长因子的水平增加，并且该药膏使脂质过氧化物，IL-6，TNF-α的水平正常化比切除受伤的动物要大。从以上结果可以得出结论，局部伤口愈合和胶原合成增加证明，局部应用草药软膏在切除伤口的大鼠中具有明显的伤口愈合活性。PDGF（血小板衍生生长因子）和EGF（表皮生长因子），脂质过氧化物（LPO），超氧化物歧化酶（SOD）以及生化参数，例如羟脯氨酸，杂糖胺和组织蛋白。药膏治疗组的局部应用表明，PDGF和EGF羟基脯氨酸，he素，组织蛋白，SOD和伤口收缩等生长因子的水平增加，并且该药膏使脂质过氧化物，IL-6，TNF-α的水平正常化比切除受伤的动物要大。从以上结果可以得出结论，局部伤口愈合和胶原合成增加证明，局部应用草药软膏在切除伤口的大鼠中具有明显的伤口愈合活性。以及生化参数，例如羟脯氨酸，半乳糖胺和组织蛋白。药膏治疗组的局部应用表明，PDGF和EGF羟基脯氨酸，he素，组织蛋白，SOD和伤口收缩等生长因子的水平增加，并且该药膏使脂质过氧化物，IL-6，TNF-α的水平正常化比切除受伤的动物要大。从以上结果可以得出结论，局部伤口愈合和胶原合成增加证明，局部应用草药软膏在切除伤口的大鼠中具有明显的伤口愈合活性。以及生化参数，例如羟脯氨酸，半乳糖胺和组织蛋白。药膏治疗组的局部应用表明，PDGF和EGF羟基脯氨酸，水苏糖胺，组织蛋白，SOD和伤口收缩等生长因子的水平增加，并且该药膏使脂质过氧化物，IL-6，TNF-α的水平正常化比切除受伤的动物要大。从以上结果可以得出结论，局部伤口愈合和胶原合成增加证明，局部应用草药软膏在切除伤口的大鼠中具有明显的伤口愈合活性。与切除伤口的动物相比，SOD和伤口收缩以及药膏使脂质过氧化物，IL-6，TNF-α的水平正常化。从以上结果可以得出结论，局部伤口愈合和胶原合成增加证明，局部应用草药软膏在切除伤口的大鼠中具有明显的伤口愈合活性。与切除伤口的动物相比，SOD和伤口收缩以及药膏使脂质过氧化物，IL-6，TNF-α的水平正常化。从以上结果可以得出结论，局部伤口愈合和胶原合成增加证明，局部应用草药软膏在切除伤口的大鼠中具有明显的伤口愈合活性。