Background. Treating nonalcoholic fatty liver disease (NAFLD) is considered one of the public health priorities in the past decade. So far, probiotics have represented promising results in controlling the signs and symptoms of NAFLD. However, attempts to find the ideal probiotic strain are still ongoing. The present study is designed to find the best strain amongst suitable probiotic strains according to their ability to ameliorate histopathological and oxidative stress biomarkers in hepatic steatosis-induced rats. Methods. Initially, four probiotics species, including Lactobacillus (L.) acidophilus, L. casei, L. reuteri, and Bacillus coagulans, were cultured and prepared as a lyophilized powder for animals. The experiment lasted for fifty days. Initially, hepatic steatosis was induced by excessive ingestion of D-fructose in rats for eight weeks, followed by eight weeks of administering probiotics and D-fructose concurrently. Forty-two six-week-old male rats were alienated to different groups and were supplemented with different probiotics ( in 500 mL drinking water). After eight weeks, blood and liver samples were taken for further evaluation, and plasma and oxidative stress markers corresponding to liver injuries were examined. Results. Administration of probiotics over eight weeks reversed hepatic and blood triglyceride concentration and blood glucose levels. Also, probiotics significantly suppressed markers of oxidative stress in the liver tissue. Conclusions. Although some of the single probiotic formulations were able to mitigate oxidative stress markers, mixtures of probiotics significantly ameliorated more symptoms in the NAFLD animals. This enhanced effect might be due to probiotics’ cumulative potential to maintain oxidative stress and deliver improved lipid profiles, liver function markers, and inflammatory markers.

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益生菌补充剂在肝脂肪变性中的保护作用：大鼠模型研究

背景。治疗非酒精性脂肪肝（NAFLD）被认为是过去十年的公共卫生重点之一。到目前为止，益生菌在控制 NAFLD 的体征和症状方面取得了可喜的成果。然而，寻找理想益生菌菌株的尝试仍在进行中。本研究旨在根据其改善肝脂肪变性诱导大鼠的组织病理学和氧化应激生物标志物的能力，在合适的益生菌菌株中找到最佳菌株。方法。最初，四种益生菌种类包括嗜酸乳杆菌( L. ) 、干酪乳杆菌、罗伊氏乳杆菌和凝结芽孢杆菌，被培养并制备为动物用冻干粉。实验持续了五十天。最初，大鼠过量摄入 D-果糖 8 周可诱发肝脂肪变性，随后同时服用益生菌和 D-果糖 8 周。将 42 只 6 周大的雄性大鼠异化到不同的组，并补充不同的益生菌（500 毫升饮用水中）。八周后，采集血液和肝脏样本进行进一步评估，并检查与肝损伤相对应的血浆和氧化应激标志物。结果。在八周内服用益生菌可逆转肝脏和血液中的甘油三酯浓度和血糖水平。此外，益生菌显着抑制了肝组织中的氧化应激标志物。结论. 尽管一些单一的益生菌制剂能够减轻氧化应激标志物，但益生菌混合物显着改善了 NAFLD 动物的更多症状。这种增强的效果可能是由于益生菌在维持氧化应激和提供改善的血脂、肝功能标志物和炎症标志物方面的累积潜力。