当前位置：
X-MOL 学术
›
Chem. Res. Toxicol.
›
论文详情
Our official English website, www.x-mol.net, welcomes your
feedback! (Note: you will need to create a separate account there.)
Electronic Cigarette Refill Fluids Sold Worldwide: Flavor Chemical Composition, Toxicity, and Hazard Analysis
Chemical Research in Toxicology ( IF 3.7 ) Pub Date : 2020-11-23 , DOI: 10.1021/acs.chemrestox.0c00266 Esther E Omaiye 1, 2 , Wentai Luo 3, 4 , Kevin J McWhirter 3 , James F Pankow 3, 4 , Prue Talbot 2
Chemical Research in Toxicology ( IF 3.7 ) Pub Date : 2020-11-23 , DOI: 10.1021/acs.chemrestox.0c00266 Esther E Omaiye 1, 2 , Wentai Luo 3, 4 , Kevin J McWhirter 3 , James F Pankow 3, 4 , Prue Talbot 2
Affiliation
|
Flavor chemicals in electronic cigarette (EC) fluids, which may negatively impact human health, have been studied in a limited number of countries/locations. To gain an understanding of how the composition and concentrations of flavor chemicals in ECs are influenced by product sale location, we evaluated refill fluids manufactured by one company (Ritchy LTD) and purchased worldwide. Flavor chemicals were identified and quantified using gas chromatography/mass spectrometry (GC/MS). We then screened the fluids for their effects on cytotoxicity (MTT assay) and proliferation (live-cell imaging) and tested authentic standards of specific flavor chemicals to identify those that were cytotoxic at concentrations found in refill fluids. A total of 126 flavor chemicals were detected in 103 bottles of refill fluid, and their number per/bottle ranged from 1–50 based on our target list. Two products had none of the flavor chemicals on our target list, nor did they have any nontargeted flavor chemicals. A total of 28 flavor chemicals were present at concentrations ≥1 mg/mL in at least one product, and 6 of these were present at concentrations ≥10 mg/mL. The total flavor chemical concentration was ≥1 mg/mL in 70% of the refill fluids and ≥10 mg/mL in 26%. For sub-brand duplicate bottles purchased in different countries, flavor chemical concentrations were similar and induced similar responses in the in vitro assays (cytotoxicity and cell growth inhibition). The levels of furaneol, benzyl alcohol, ethyl maltol, ethyl vanillin, corylone, and vanillin were significantly correlated with cytotoxicity. The margin of exposure calculations showed that pulegone and estragole levels were high enough in some products to present a nontrivial calculated risk for cancer. Flavor chemical concentrations in refill fluids often exceeded concentrations permitted in other consumer products. These data support the regulation of flavor chemicals in EC products to reduce their potential for producing both cancer and noncancer toxicological effects.
中文翻译：
全球销售的电子烟补充液：香料化学成分、毒性和危害分析
电子烟 (EC) 液体中的香料化学物质可能会对人类健康产生负面影响，已在少数国家/地区进行了研究。为了了解 EC 中风味化学品的成分和浓度如何受到产品销售地点的影响，我们评估了一家公司 (Ritchy LTD) 生产并在全球范围内购买的补充液。使用气相色谱/质谱 (GC/MS) 来鉴定和量化风味化学物质。然后，我们筛选了这些液体对细胞毒性（MTT 测定）和增殖（活细胞成像）的影响，并测试了特定风味化学物质的真实标准，以确定补充液体中发现的浓度具有细胞毒性的化学物质。在 103 瓶补充液中总共检测到 126 种香料化学物质，根据我们的目标清单，每瓶的数量在 1 至 50 种之间。两种产品不含有我们目标清单上的任何风味化学品，也不含有任何非目标风味化学品。至少一种产品中总共 28 种风味化学品的浓度≥1 mg/mL，其中 6 种的浓度≥10 mg/mL。 70% 的补充液中总香料化学浓度≥1 mg/mL，26% 的补充液中总香料化学浓度≥10 mg/mL。对于在不同国家购买的子品牌重复瓶子，风味化学物质浓度相似，并在体外测定中诱导相似的反应（细胞毒性和细胞生长抑制）。呋喃酚、苯甲醇、乙基麦芽酚、乙基香兰素、紫堇酮和香兰素的水平与细胞毒性显着相关。暴露范围计算表明，某些产品中胡薄荷酮和草蒿脑的含量足够高，可以计算得出不小的癌症风险。 填充液中的香料化学浓度通常超过其他消费品允许的浓度。这些数据支持对 EC 产品中的风味化学品进行监管，以减少其产生癌症和非癌症毒理学作用的可能性。
更新日期：2020-12-21
中文翻译：
全球销售的电子烟补充液：香料化学成分、毒性和危害分析
电子烟 (EC) 液体中的香料化学物质可能会对人类健康产生负面影响，已在少数国家/地区进行了研究。为了了解 EC 中风味化学品的成分和浓度如何受到产品销售地点的影响，我们评估了一家公司 (Ritchy LTD) 生产并在全球范围内购买的补充液。使用气相色谱/质谱 (GC/MS) 来鉴定和量化风味化学物质。然后，我们筛选了这些液体对细胞毒性（MTT 测定）和增殖（活细胞成像）的影响，并测试了特定风味化学物质的真实标准，以确定补充液体中发现的浓度具有细胞毒性的化学物质。在 103 瓶补充液中总共检测到 126 种香料化学物质，根据我们的目标清单，每瓶的数量在 1 至 50 种之间。两种产品不含有我们目标清单上的任何风味化学品，也不含有任何非目标风味化学品。至少一种产品中总共 28 种风味化学品的浓度≥1 mg/mL，其中 6 种的浓度≥10 mg/mL。 70% 的补充液中总香料化学浓度≥1 mg/mL，26% 的补充液中总香料化学浓度≥10 mg/mL。对于在不同国家购买的子品牌重复瓶子，风味化学物质浓度相似，并在体外测定中诱导相似的反应（细胞毒性和细胞生长抑制）。呋喃酚、苯甲醇、乙基麦芽酚、乙基香兰素、紫堇酮和香兰素的水平与细胞毒性显着相关。暴露范围计算表明，某些产品中胡薄荷酮和草蒿脑的含量足够高，可以计算得出不小的癌症风险。 填充液中的香料化学浓度通常超过其他消费品允许的浓度。这些数据支持对 EC 产品中的风味化学品进行监管，以减少其产生癌症和非癌症毒理学作用的可能性。
京公网安备 11010802027423号