Oriented Immobilization of Protein Templates: A New Trend in Surface Imprinting,ACS Sensors

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Oriented Immobilization of Protein Templates: A New Trend in Surface Imprinting
ACS Sensors ( IF 8.2 ) Pub Date : 2020-11-22 , DOI: 10.1021/acssensors.0c01634
Jakub Kalecki ₁ , Zofia Iskierko ₁ , Maciej Cieplak ₁ , Piyush S Sharma ₁

Affiliation

In this Review, we have summarized recent trends in protein template imprinting. We emphasized a new trend in surface imprinting, namely, oriented protein immobilization. Site-directed proteins were assembled through specially selected functionalities. These efforts resulted in a preferably oriented homogeneous protein construct with decreased protein conformation changes during imprinting. Moreover, the maximum functionality for protein recognition was utilized. Various strategies were exploited for oriented protein immobilization, including covalent immobilization through a boronic acid group, metal coordinating center, and aptamer-based immobilization. Moreover, we have discussed the involvement of semicovalent as well as covalent imprinting. Interestingly, these approaches provided additional recognition sites in the molecular cavities imprinted. Therefore, these molecular cavities were highly selective, and the binding kinetics was improved.

中文翻译：

蛋白质模板的定向固定：表面印迹的新趋势

在这篇综述中，我们总结了蛋白质模板印迹的最新趋势。我们强调了表面印迹的新趋势，即定向蛋白质固定化。定点蛋白质是通过特别选择的功能来组装的。这些努力产生了优选定向的均质蛋白质构建体，在印记过程中减少了蛋白质构象变化。此外，还利用了蛋白质识别的最大功能。采用了多种策略进行定向蛋白质固定，包括通过硼酸基团的共价固定、金属配位中心和基于适体的固定。此外，我们还讨论了半共价和共价印记的参与。有趣的是，这些方法在印迹的分子腔中提供了额外的识别位点。因此，这些分子空腔具有高度选择性，并且结合动力学得到改善。

更新日期：2020-12-24

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