Vector competence refers to the ability of a vector to acquire, maintain, and transmit a pathogen. Collecting mosquito saliva in medium-filled capillary tubes has become the standard for approximating arbovirus transmission. However, this method is time-consuming and labor-intensive. Here we compare the capillary tube method to an alternative high-throughput detection method the collection of saliva on paper cards saturated with honey, with (FTA card) and without (filter paper) reagents for the preservation of nucleic acid for Aedes aegypti and Aedes albopictus mosquitoes infected with two emerging genotypes of the chikungunya virus (CHIKV). Model results showed that the Asian genotype CHIKV dissemination in the harvested legs of both Ae. aegypti and Ae. albopictus increased the odds of females having a positive salivary infection and higher salivary viral titers, while for the IOL genotype the same effect was observed only for Ae. aegypti. Of the three tested detection methods, the FTA card was significantly more effective at detecting infected saliva of Ae. aegypti and Ae. albopictus females than the capillary tube and filter paper was as effective as the capillary tube for the Asian genotype. We did not find significant effects of the detection method in detecting higher viral titer for both Asian and IOL genotypes. Our results are discussed in light of the limitations of the different tested detection methods.

中文翻译：

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高通量方法检测埃及伊蚊和埃及伊蚊唾液中虫媒病毒感染。白化病

载体能力是指载体获取，维持和传播病原体的能力。在中等填充的毛细管中收集蚊子唾液已成为近似虫媒病毒传播的标准。但是，这种方法既费时又费力。在这里，我们将毛细管法与另一种高通量检测方法进行比较，该方法是在充满蜂蜜的纸卡上收集唾液，使用（FTA卡）和不使用（滤纸）试剂保存埃及伊蚊和白纹伊蚊的核酸蚊感染了基孔肯雅热病毒（CHIKV）的两种新出现的基因型。模型结果表明，亚洲基因型CHIKV在两个Ae的收获的腿中传播。埃及和e 白化病增加了唾液感染阳性和唾液病毒滴度较高的雌性的几率，而对于IOL基因型，仅对Ae观察到了相同的效果。伊蚊。在三种测试的检测方法中，FTA卡在检测受感染的Ae唾液方面明显更有效。埃及和埃及。白喉女性比毛细管和滤纸对亚洲基因型的效果还好于毛细管。我们没有发现该检测方法在检测亚洲和IOL基因型的更高病毒滴度中的显着效果。根据不同测试方法的局限性讨论了我们的结果。