Deficiency of selenium (Se) will lead to malnutrition and decreased immune function of the body. There is a common phenomenon of Se deficiency in foods. In this study, different concentrations of sodium selenite (Na₂SeO₃) were applied to Moringa oleifera grownin soil. The purpose was to explore the feasibility of Se biofortification of M. oleifera root. The effect of exogenous Se on the accumulation of Se and cadmium (Cd) in the roots of M. oleifera was studied by inductively coupled plasma mass spectrometry, and the mechanism of exogenous Se on the accumulation of Se and Cd in the roots was studied by Fourier transform infrared spectroscopy (FTIR) combined with principal component analysis and partial least squares regression analysis. The results showed that Na₂SeO₃ significantly affected the accumulation of Se and Cd in the roots (p < 0.05). The increase in Se was highest when Na₂SeO₃ was around 4.0 mg/kg, which increased by 315% compared with the control. The decrease in Cd was the lowest when Na₂SeO₃ was around 2.0 mg/kg, which decreased by 80% compared with the control. The results of FTIR analysis showed that Na₂SeO₃ treatment changed the carboxylate, phosphate radical, hemicellulose and protein in roots of M. oleifera, while the increase of Se was related to hemicellulose, protein, polysaccharide and lignin, and the decrease of Cd was related to hemicellulose and protein. The results showed that exogenous Se increased the accumulation of Se and inhibited the absorption of Cd. Therefore, the roots of M. oleifera can be used in Se biofortified products.

硒（Se）缺乏会导致营养不良和机体免疫功能下降。食物中普遍存在硒缺乏症。在这项研究中，将不同浓度亚硒酸钠（钠的₂的SeO ₃）施加于辣木grownin土壤。目的是探讨油橄榄分枝杆菌根系硒生物强化的可行性。外源硒对油茶分枝根中硒和镉（Cd）积累的影响通过电感耦合等离子体质谱法研究了硒，并通过傅里叶变换红外光谱（FTIR）结合主成分分析和偏最小二乘回归分析研究了外源硒对根中硒和镉积累的机理。结果表明，Na ₂ SeO ₃显着影响根系中硒和镉的积累（p  <0.05）。当Na ₂ SeO ₃约为4.0 mg / kg时，Se的增加最高，与对照相比增加了315％。当Na ₂ SeO ₃时，Cd的减少最低。浓度约为2.0 mg / kg，与对照组相比下降了80％。FTIR分析结果表明，Na ₂ SeO ₃处理改变了油茶根中的羧酸根，磷酸根，半纤维素和蛋白质，而硒的增加与半纤维素，蛋白质，多糖和木质素，Cd的减少有关。与半纤维素和蛋白质有关。结果表明，外源硒增加了硒的积累，抑制了镉的吸收。因此，M.oleifera的根可用于Se生物强化产品。