Osteoclasts (OCs) and much less dendritic cells (DCs) induce significant expansion and functional activation of NK cells, and furthermore, the OC-expanded NK cells preferentially increase the expansion and activation of CD8+ T cells by targeting CD4+ T cells. When autologous OCs were used to expand patient NK cells much lower percentages of expanded CD8+ T cells, decreased numbers of expanded NK cells and decreased functions of NK cells could be observed, and the addition of allogeneic healthy OCs increased the patients’ NK function. Mechanistically, OC-expanded NK cells were found to lyse CD4+ T cells but not CD8+ T cells suggesting potential selection of CD8+ T cells before their expansion by OC activated NK cells. In agreement, Increased IFN-γ secretion, and NK cell-mediated cytotoxicity and higher percentages of CD8+ T cells, in various tissue compartments of oral tumor-bearing hu-BLT mice in response to immunotherapy by OC-expanded NK cells were observed. Thus, our results indicate an important relationship between NK and CD8+ T cells.

破骨细胞（OC）和更少的树突状细胞（DC）诱导NK细胞的显着扩增和功能激活，此外，OC扩增的NK细胞通过靶向CD4 + T细胞优先增加CD8 + T细胞的扩增和激活。当使用自体 OC 扩增患者 NK 细胞时，扩增的 CD8+ T 细胞的百分比要低得多，可以观察到扩增的 NK 细胞数量减少和 NK 细胞功能下降，而添加同种异体健康 OC 则增加了患者的 NK 功能。从机制上讲，OC 扩增的 NK 细胞被发现裂解 CD4+ T 细胞，但不裂解 CD8+ T 细胞，这表明在 OC 激活的 NK 细胞扩增之前可能会选择 CD8+ T 细胞。一致地，在口腔肿瘤 hu-BLT 小鼠的各个组织区室中，观察到对 OC 扩增的 NK 细胞免疫治疗的反应增加了 IFN-γ 分泌、NK 细胞介导的细胞毒性和更高的 CD8+ T 细胞百分比。因此，我们的结果表明 NK 和 CD8+ T 细胞之间存在重要关系。