One current goal in native mass spectrometry is the assignment of binding affinities to noncovalent complexes. Here we introduce a novel implementation of the existing laser-induced liquid bead ion desorption (LILBID) mass spectrometry method: this new method, LILBID laser dissociation curves, assesses binding strengths quantitatively. In all LILBID applications, aqueous sample droplets are irradiated by 3 µm laser pulses. Variation of the laser energy transferred to the droplet during desorption affects the degree of complex dissociation. In LILBID laser dissociation curves, laser energy transfer is purposely varied, and a binding affinity is calculated from the resulting complex dissociation. A series of dsDNAs with different binding affinities was assessed using LILBID laser dissociation curves. The binding affinity results from the LILBID laser dissociation curves strongly correlated with the melting temperatures from UV melting curves and with dissociation constants from isothermal titration calorimetry, standard solution phase methods. LILBID laser dissociation curve data also showed good reproducibility and successfully predicted the melting temperatures and dissociation constants of three DNA sequences. LILBID laser dissociation curves are a promising native mass spectrometry binding affinity method, with reduced time and sample consumption compared to melting curves or titrations.

天然质谱的当前目标之一是将结合亲和力分配给非共价复合物。在这里，我们介绍了现有的激光诱导液珠离子解吸（LILBID）质谱方法的一种新颖实现：这种新方法，即LILBID激光解离曲线，可以定量评估结合强度。在所有LILBID应用中，都通过3 µm激光脉冲辐照含水样品滴。解吸过程中转移到液滴上的激光能量的变化会影响复合物的解离程度。在LILBID激光解离曲线中，有目的地改变激光能量传递，并根据所得的复杂解离计算出结合亲和力。使用LILBID激光解离曲线评估了一系列具有不同结合亲和力的dsDNA。LILBID激光解离曲线的结合亲和力与UV熔融曲线的解链温度，等温滴定量热法，标准溶液相方法的解离常数高度相关。LILBID激光解离曲线数据也显示出良好的重现性，并成功预测了三个DNA序列的解链温度和解离常数。LILBID激光解离曲线是一种很有前途的天然质谱结合亲和方法，与熔解曲线或滴定法相比，可减少时间和样品消耗。LILBID激光解离曲线数据也显示出良好的重现性，并成功预测了三个DNA序列的解链温度和解离常数。LILBID激光解离曲线是一种很有前途的天然质谱结合亲和方法，与熔解曲线或滴定法相比，具有减少的时间和样品消耗。LILBID激光解离曲线数据也显示出良好的重现性，并成功预测了三个DNA序列的解链温度和解离常数。LILBID激光解离曲线是一种很有前途的天然质谱结合亲和方法，与熔解曲线或滴定法相比，可减少时间和样品消耗。