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Hierarchical Gold Mesoflowers in Enzyme Engineering: An Environmentally Friendly Strategy for the Enhanced Enzymatic Performance and Biodiesel Production
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2020-11-23 , DOI: 10.1021/acsabm.0c00721 Saghar Rezaei 1 , Amir Landarani-Isfahani 1 , Majid Moghadam 1 , Shahram Tangestaninejad 1 , Valiollah Mirkhani 1 , Iraj Mohammadpoor-Baltork 1
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2020-11-23 , DOI: 10.1021/acsabm.0c00721 Saghar Rezaei 1 , Amir Landarani-Isfahani 1 , Majid Moghadam 1 , Shahram Tangestaninejad 1 , Valiollah Mirkhani 1 , Iraj Mohammadpoor-Baltork 1
Affiliation
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To expand the field of nanomaterial and engineering of enzyme in eco-friendly processes, gold mesoflower (Au-MF) nanostructure was applied for preparation of three series of immobilized lipase (Au-MF/SAM 1–3) through biofunctionalization of surface by Ugi multicomponent reaction. The synthesized Au-MF/SAM 1–3/lipase as unique biocatalysts was confirmed by different analytical tools and techniques. Compared to the free lipase, the Au-MF/SAM 1–3/lipase showed more stability at high temperature and pH. Also, these biocatalysts showed high storage stability and reusability after 2 months and eight cycles, respectively. Moreover, the kinetic behavior was investigated and the results showed a minimal impairment of catalytic activity of immobilized lipase. The kinetic constants of the immobilized lipase, Au-MF/SAM 2/lipase, are Km = 0.37 mM, Vmax = 0.22 mM min–1, and kcat = 154 min–1. The immobilized lipase showed smaller activation energy (Ea) than that of free enzyme, indicating that the immobilized enzyme is less sensitive to temperature. In the following, the biodiesel production from palmitic acid was studied in the presence of Au-MF/SAM 2/lipase as an efficient biocatalyst. The influence of different reaction parameters such as temperature, molar ratio of alcohol to palmitic acid, water content, and lipase amount was deeply investigated.
中文翻译：
酶工程中的分级金中花：增强酶促性能和生物柴油生产的环保策略
为了拓展纳米材料和酶工程在环保过程中的应用领域，金中花（Au-MF）纳米结构被应用于通过 Ugi 表面的生物功能化制备三个系列的固定化脂肪酶（Au-MF/SAM 1-3）多组分反应。不同的分析工具和技术证实了合成的 Au-MF/SAM 1-3 /脂肪酶作为独特的生物催化剂。与游离脂肪酶相比，Au-MF/SAM 1 – 3/脂肪酶在高温和 pH 值下表现出更高的稳定性。此外，这些生物催化剂分别在 2 个月和 8 个循环后显示出高储存稳定性和可重复使用性。此外，研究了动力学行为，结果表明固定化脂肪酶的催化活性受损最小。固定化脂肪酶 Au-MF/SAM 2/脂肪酶的动力学常数为K m = 0.37 mM、V max = 0.22 mM min –1和k cat = 154 min –1。固定化脂肪酶显示出较小的活化能（E a) 比游离酶低，表明固定化酶对温度的敏感性较低。在下文中，研究了在作为有效生物催化剂的 Au-MF/SAM 2 /脂肪酶存在下从棕榈酸生产生物柴油。深入研究了温度、醇与棕榈酸的摩尔比、含水量和脂肪酶用量等不同反应参数的影响。
更新日期：2020-12-21
中文翻译：
酶工程中的分级金中花：增强酶促性能和生物柴油生产的环保策略
为了拓展纳米材料和酶工程在环保过程中的应用领域，金中花（Au-MF）纳米结构被应用于通过 Ugi 表面的生物功能化制备三个系列的固定化脂肪酶（Au-MF/SAM 1-3）多组分反应。不同的分析工具和技术证实了合成的 Au-MF/SAM 1-3 /脂肪酶作为独特的生物催化剂。与游离脂肪酶相比，Au-MF/SAM 1 – 3/脂肪酶在高温和 pH 值下表现出更高的稳定性。此外，这些生物催化剂分别在 2 个月和 8 个循环后显示出高储存稳定性和可重复使用性。此外，研究了动力学行为，结果表明固定化脂肪酶的催化活性受损最小。固定化脂肪酶 Au-MF/SAM 2/脂肪酶的动力学常数为K m = 0.37 mM、V max = 0.22 mM min –1和k cat = 154 min –1。固定化脂肪酶显示出较小的活化能（E a) 比游离酶低，表明固定化酶对温度的敏感性较低。在下文中，研究了在作为有效生物催化剂的 Au-MF/SAM 2 /脂肪酶存在下从棕榈酸生产生物柴油。深入研究了温度、醇与棕榈酸的摩尔比、含水量和脂肪酶用量等不同反应参数的影响。
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