ABSTRACT The ornamental plant Camellia japonica is widely distributed worldwide and is susceptible to various environmental stresses. The WRKY transcription factor (TF) is an important node of plant tolerance. However, WRKY TFs from C. japonica have not been reported yet. In this study, 48 CjWRKYs, namely, CjWRKY1 to CjWRKY48, were identified. Protein structure analysis revealed that CjWRKY proteins contain a highly conserved motif (WRKYGQK) and two variant motifs (WRKYGKK and WRKYGRK). Phylogenetic analysis indicated that the 48 CjWRKYs can be divided into three groups, which are further classified into six subgroups, namely, I-C, II-a, II-b, II-c, II-e, and III, which contain 10, 6, 8, 13, 7, and 4 members, respectively. The expression patterns of 15 CjWRKYs under salicylic acid (SA) treatment were investigated by real-time quantitative PCR (qRT-PCR). Results showed that the 15 CjWRKYs could be induced by SA treatment. This study is the first to screen CjWRKYs and identify the expression profile of CjWRKYs under SA treatment and provides a theoretical basis for analyzing the function of CjWRKY genes to SA stress tolerance in C. japonica.

中文翻译：

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WRKY 家族基因的全转录组鉴定及其对山茶中水杨酸的表达谱

摘要 观赏植物山茶在世界范围内广泛分布，易受各种环境胁迫的影响。WRKY 转录因子 (TF) 是植物耐受性的重要节点。然而，尚未报道来自 C. japonica 的 WRKY TF。在这项研究中，鉴定了 48 个 CjWRKY，即 CjWRKY1 到 CjWRKY48。蛋白质结构分析表明，CjWRKY 蛋白质包含一个高度保守的基序（WRKYGQK）和两个变异基序（WRKYGKK 和 WRKYGRK）。系统发育分析表明，48个CjWRKYs可分为3类，进一步分为IC、II-a、II-b、II-c、II-e和III 6个亚类，其中包含10、6 、 8、 13、 7 和 4 名成员，分别。通过实时定量 PCR (qRT-PCR) 研究了 15 个 CjWRKY 在水杨酸 (SA) 处理下的表达模式。结果表明SA处理可诱导15个CjWRKYs。本研究首次筛选CjWRKYs并鉴定SA处理下CjWRKYs的表达谱，为分析CjWRKY基因对粳稻SA胁迫耐受性的功能提供理论依据。