Acrylamide is an important monomer for the synthesis of polyacrylamide, which has wide applications in industries. This molecule can be produced by the hydration of acrylonitrile, catalyzed by the enzyme nitrile hydratase (NHase) secreted by microorganisms. Herein, synthesis of acrylamide was carried out by soil isolate Rhodococcus rhodochrous (RS‐6) contains NHase. The critical parameters affecting the bioconversion process such as pH, temperature, substrate concentration, resting cell concentration and acrylamide concentration were optimized by changing one parameter at a time.

中文翻译：

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杜鹃红球菌（RS-6）的腈水合酶介导的丙烯腈转化

丙烯酰胺是合成聚丙烯酰胺的重要单体，在工业上具有广泛的应用。该分子可通过由微生物分泌的腈水合酶（NHase）催化丙烯腈水合而产生。在这里，丙烯酰胺的合成是通过含有NHase的土壤分离株Rhodococcus rhodochrous（RS-6）进行的。通过一次更改一个参数来优化影响生物转化过程的关键参数，例如pH，温度，底物浓度，静止细胞浓度和丙烯酰胺浓度。