AP‐3 (adaptor complex 3) mediates traffic from the late Golgi or early endosomes to late endosomal compartments. In mammals, mutations in AP‐3 cause Hermansky‐Pudlak syndrome type 2, cyclic neutropenias, and a form of epileptic encephalopathy. In budding yeast, AP‐3 carries cargo directly from the trans‐Golgi to the lysosomal vacuole. Despite the pathway's importance and its discovery two decades ago, rapid screens and selections for AP‐3 mutants have not been available. We now report GNSI, a synthetic, genetically encoded reporter that allows rapid plate‐based assessment of AP‐3 functional deficiency, using either chromogenic or growth phenotype readouts. This system identifies defects in both the formation and consumption of AP‐3 carrier vesicles and is adaptable to high‐throughput screening or selection in both plate array and liquid batch culture formats. Episomal and integrating plasmids encoding GNSI have been submitted to the Addgene repository.

中文翻译：

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芽孢酵母中衔接子复合体3的遗传多态报告基因

AP-3（适配器复合物3）介导从高尔基晚期或早期内体到晚期内体区室的运输。在哺乳动物中，AP-3的突变会导致2型赫曼斯基-普德拉克综合征，周期性中性粒细胞减少和一种癫痫性脑病。在发芽酵母中，AP-3将货物直接从反式高尔基体运送到溶酶体液泡中。尽管该途径的重要性和二十年前的发现，但尚未提供针对AP-3突变体的快速筛选和选择。现在，我们报告GNSI，这是一种合成的，遗传编码的报告基因，可使用发色或生长表型读数对AP-3功能缺陷进行基于板的快速评估。该系统可识别AP-3载体囊泡的形成和消耗中的缺陷，并且适用于平板阵列和液体分批培养形式的高通量筛选或选择。编码GNSI的附加型和整合型质粒已提交至Addgene信息库。