The LRK10-like receptor kinases (LRK10L-RLKs) are ubiquitously present in higher plants, but knowledge of their expression and function is still limited. Here, we report expression and functional analysis of TtdLRK10L-1, a typical LRK10L-RLK in durum wheat (Triticum turgidum L. ssp. durum). The introns of TtdLRK10L-1 contained multiple kinds of predicted cis-elements. To investigate the potential effect of these cis-elements on TtdLRK10L-1 expression and function, two types of transgenic wheat lines were prepared, which expressed a GFP-tagged TtdLRK10L-1 protein (TtdLRK10L-1:GFP) from the cDNA or genomic DNA (gDNA) sequence of TtdLRK10L-1 under the native promoter. TtdLRK10L-1:GFP expression was up-regulated by the powdery mildew pathogen Blumeria graminis f. sp. tritici (Bgt) in both types of transgenic plants, with the scale of the elevation being much stronger in the gDNA lines. Both types of transgenic plants exhibited enhanced resistance to Bgt infection relative to wild type control. Notably, the Bgt defence activated in the gDNA lines was significantly stronger than that in the cDNA lines. Further analysis revealed that a putative MYB transcription factor binding site (MYB-BS, CAGTTA) located in TtdLRK10L-1 intron I was critical for the efficient expression and function of TtdLRK10L-1 in Bgt defence. This MYB-BS could also increase the activity of a superpromoter widely used in ectopic gene expression studies in plants. Together, our results deepen the understanding of the expression and functional characteristics of LRK10L-RLKs. TtdLRK10L-1 is likely useful for further dissecting the molecular processes underlying wheat defence against Bgt and for developing Bgt resistant wheat crops.

中文翻译：

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小麦白粉病防御中受体样激酶的有效表达和功能需要内含子定位的MYB结合位点

LRK10样受体激酶（LRK10L-RLK）普遍存在于高等植物中，但对其表达和功能的了解仍然有限。这里，我们报告表达和TtdLRK10L-1，典型的LRK10L-RLK在硬粒小麦（功能分析圆柱小麦L. SSP。硬粒小麦）。TtdLRK10L-1的内含子包含多种预测的顺式元素。为了研究这些顺式元素对TtdLRK10L-1表达和功能的潜在影响，制备了两种类型的转基因小麦品系，它们从cDNA或基因组DNA表达GFP标记的TtdLRK10L-1蛋白（TtdLRK10L-1：GFP）。TtdLRK10L-1的（gDNA）序列在本地启动子下。TtdLRK10L-1：GFP的表达被白粉病病原体Blumeria graminis f上调。sp。两种转基因植物中都存在小麦（Bgt），而在gDNA系中海拔高度要强得多。相对于野生型对照，两种类型的转基因植物均显示出对Bgt感染的增强的抗性。值得注意的是，在gDNA系中激活的Bgt防御明显强于cDNA系。进一步的分析表明，位于TtdLRK10L-1内含子I上的推测的MYB转录因子结合位点（MYB-BS，CAGTTA）对于TgtdLRK10L-1在Bgt中的有效表达和功能至关重要。防御。该MYB-BS还可以增加在植物异位基因表达研究中广泛使用的超级启动子的活性。在一起，我们的结果加深​​了对LRK10L-RLK的表达和功能特性的理解。TtdLRK10L-1可能有助于进一步剖析小麦防御Bgt的分子过程，以及开发抗Bgt小麦作物。