Psoriasis is a chronic immune‐mediated skin disease accompanied by systemic inflammation and comorbidities. We analyzed peripheral blood mononuclear cells (PBMCs) in the search for immune signatures and biomarkers related to psoriasis severity and treatment effect. Thirty‐two patients with psoriasis and 10 matched healthy controls were included. PBMCs were collected before and after initiation of anti‐TNF, anti‐IL‐17 or anti‐IL‐12/23 treatment and analyzed utilizing 26‐parameter mass cytometry. The number of circulating Th17, Th22, Th9, and cytotoxic T cells were increased in severe psoriasis. Intracellular pp38 and pERK in T helper cells were associated with disease severity. Differences between responders and nonresponders regarding cell composition and intracellular signaling were identifiable already at inclusion. Biological treatment induced memory cells, restored inhibitory PD‐1 function of T cells, and reduced a potential pro‐atherogenic profile in monocytes. In conclusion, these results indicate amelioration of systemic inflammation in psoriasis after biological treatment. Such broad immune profiling may enable prospective stratification of patients regarding future treatment response. Successful early intervention may lead to a healthier trajectory with favorable implications on later comorbidities.

中文翻译：

---

生物治疗银屑病患者血液免疫细胞的质谱分析

银屑病是一种慢性免疫介导的皮肤病，伴有全身炎症和合并症。我们分析了外周血单核细胞 (PBMC)，以寻找与银屑病严重程度和治疗效果相关的免疫特征和生物标志物。包括 32 名银屑病患者和 10 名匹配的健康对照。在开始抗 TNF、抗 IL-17 或抗 IL-12/23 治疗之前和之后收集 PBMC，并使用 26 参数质谱仪进行分析。重度银屑病中循环 Th17、Th22、Th9 和细胞毒性 T 细胞的数量增加。T辅助细胞中的细胞内pp38和pERK与疾病严重程度相关。反应者和非反应者之间在细胞组成和细胞内信号传导方面的差异在纳入时已经可以识别。生物治疗可诱导记忆细胞，恢复 T 细胞的抑制性 PD-1 功能，并降低单核细胞中潜在的促动脉粥样硬化特征。总之，这些结果表明生物治疗后银屑病的全身炎症得到改善。这种广泛的免疫分析可以使患者对未来的治疗反应进行前瞻性分层。成功的早期干预可能会导致更健康的轨迹，并对以后的合并症产生有利影响。