Interspecies interactions are key determinants in biofilm behavior, ecology, and architecture. The cellular responses of microorganisms to each other at transcriptional, proteomic, and metabolomic levels ultimately determine the characteristics of biofilm and the corresponding implications for health and disease. Advances in omics technologies have revolutionized our understanding of microbial community composition and their activities as a whole. Large‐scale analyses of the complex interaction between the many microbial species residing within a biofilm, however, are currently still hampered by technical and bioinformatics challenges. Thus, studies of interspecies interactions have largely focused on the transcriptional and proteomic changes that occur during the contact of a few prominent species, such as Porphyromonas gingivalis, Streptococcus mutans, Candida albicans, and a few others, with selected partner species. Expansion of available tools is necessary to grow the revealing, albeit limited, insight these studies have provided into a profound understanding of the nature of individual microbial responses to the presence of others. This will allow us to answer important questions including: Which intermicrobial interactions orchestrate the myriad of cooperative, synergistic, antagonistic, manipulative, and other types of relationships and activities in the complex biofilm environment, and what are the implications for oral health and disease?

中文翻译：

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组学和种间相互作用

种间相互作用是生物膜行为、生态和建筑的关键决定因素。微生物在转录、蛋白质组学和代谢组学水平上的细胞反应最终决定了生物膜的特征以及对健康和疾病的相应影响。组学技术的进步彻底改变了我们对微生物群落组成及其整体活动的理解。然而，对生物膜内许多​​微生物物种之间复杂相互作用的大规模分析目前仍受到技术和生物信息学挑战的阻碍。因此，对种间相互作用的研究主要集中在几个主要物种接触期间发生的转录和蛋白质组学变化，例如牙龈卟啉单胞菌、变形链球菌、白色念珠菌和其他一些，以及选定的伙伴物种。有必要扩展可用工具，以增加这些研究提供的启发性（尽管有限）的洞察力，以深入了解个体微生物对其存在的反应的性质。这将使我们能够回答重要问题，包括：哪些微生物间相互作用在复杂的生物膜环境中协调了无数的合作、协同、对抗、操纵和其他类型的关系和活动，以及对口腔健康和疾病的影响是什么？