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Reference values for serum chemistry of wild Amazon river dolphins (Inia geoffrensis) from the central Amazon
Marine Mammal Science ( IF 2.0 ) Pub Date : 2020-11-23 , DOI: 10.1111/mms.12765
Daniela M. D. Mello 1 , Fabiana A. Melo 2 , Vera M. F. Silva 1
Affiliation  

Blood values of wild cetacean population are a valuable tool to drive proper management of threatened species. Reference intervals of 26 serum analytes were determined from 107 apparently healthy Amazon river dolphins (Inia geoffrensis) wild‐caught in the Mamirauá Sustainable Development Reserve (3°3′S, 64°51′W), central Amazon, Brazil. No differences were detected between males and females or between pregnant and nonpregnant females. Calves had higher serum calcium than juveniles and adults, and alkaline phosphatase activity was higher in calves and juveniles than adults as a result of bone growth. Adults showed higher creatinine levels than juveniles due to higher body mass, and higher urea than calves. Positive correlation was found between body length and body weight with creatinine, urea, and alanine aminotransferase activity; whereas calcium and alkaline phosphatase and creatine kinase (CK) activities negatively correlated with these body parameters. Cardiac rate and respiratory frequency appear to have not correlated with any serum analyte, while stress level positively correlated with CK. Storage of frozen samples for 60 days at −80°C significantly altered 10 of the 19 analytes after one freeze–thaw cycle. Important differences were detected among age classes, stress level, and sample storage and should be taken into account before any clinical or physiological interpretations.

中文翻译:

来自亚马逊中部的野生亚马逊河海豚(Inia geoffrensis)血清化学的参考值

野生鲸类种群的血液价值是推动对受威胁物种进行适当管理的宝贵工具。从107种看似健康的亚马逊河海豚(Inia geoffrensis)确定了26种血清分析物的参考间隔)在巴西中部亚马逊地区的Mamirauá可持续发展保护区(南纬3°3′,西经64°51′)中捕获。男性与女性之间或孕妇与非孕妇之间均未发现差异。由于骨骼的生长,犊牛的血清钙水平高于少年和成年人,而碱性磷酸酶的活性高于少年和成年人。由于较高的体重,成年人的肌酐水平高于青少年,而尿素的含量高于犊牛。体重和体重与肌酐,尿素和丙氨酸氨基转移酶活性之间呈正相关。而钙和碱性磷酸酶和肌酸激酶(CK)的活动与这些身体参数负相关。心率和呼吸频率似乎与任何血清分析物均不相关,而压力水平与CK呈正相关。在一个冻融循环后,将冷冻样品在-80°C下保存60天会显着改变19种分析物中的10种。在年龄类别,压力水平和样本存储之间检测到重要差异,在进行任何临床或生理解释之前应考虑到这些差异。
更新日期:2020-11-23
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